scGR-seq: Integrated analysis of glycan and RNA in single cells

Haruki Odaka; Haruka Ozaki; Hiroaki Tateno

doi:10.1016/j.xpro.2022.101179

scGR-seq: Integrated analysis of glycan and RNA in single cells

STAR Protoc. 2022 Feb 18;3(1):101179. doi: 10.1016/j.xpro.2022.101179. eCollection 2022 Mar 18.

Authors

Haruki Odaka¹, Haruka Ozaki², Hiroaki Tateno^{1

3}

Affiliations

¹ Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
² Bioinformatics Laboratory, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan.
³ JST PRESTO, Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.

Abstract

Glycans are structurally diverse molecules found on the surface of living cells. The protocol details a system developed for combined analysis of glycan and RNA in single cells (scGR-seq) using human induced pluripotent stem cells (hiPSCs) and hiPSC-derived neural progenitor cells (NPCs). scGR-seq consists of DNA-barcoded lectin-based glycan profiling by sequencing (scGlycan-seq) and single-cell transcriptome profiling (scRNA-seq). scGR-seq will be an essential technique to delineate the cellular heterogeneity of glycans across multicellular systems. For complete details on the use and execution of this profile, please refer to Minoshima et al. (2021).

Keywords: Cell Biology; Cell culture; Genomics; RNAseq; Sequencing; Single Cell.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Humans
Induced Pluripotent Stem Cells*
Polysaccharides
RNA* / genetics
Single-Cell Analysis / methods
Transcriptome / genetics

Substances

Polysaccharides
RNA