Efficiently unsterile polyhydroxyalkanoate production from lignocellulose by using alkali-halophilic Halomonas alkalicola M2

Chao-Bing Luo; Hai-Chao Li; De-Qiang Li; Haq Nawaz; Ting-Ting You; Feng Xu

doi:10.1016/j.biortech.2022.126919

Efficiently unsterile polyhydroxyalkanoate production from lignocellulose by using alkali-halophilic Halomonas alkalicola M2

Bioresour Technol. 2022 May:351:126919. doi: 10.1016/j.biortech.2022.126919. Epub 2022 Feb 28.

Authors

Chao-Bing Luo¹, Hai-Chao Li², De-Qiang Li³, Haq Nawaz⁴, Ting-Ting You⁵, Feng Xu⁶

Affiliations

¹ Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, PR China. Electronic address: lcb911002@bjfu.edu.cn.
² Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, PR China. Electronic address: lihaichao96@bjfu.edu.cn.
³ Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, PR China. Electronic address: lsx20131120a@163.com.
⁴ Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, PR China. Electronic address: haqnawaz@bjfu.edu.cn.
⁵ Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, PR China. Electronic address: youtingting0928@bjfu.edu.cn.
⁶ Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, PR China; Shandong Key Laboratory of Paper Science & Technology, Qilu University of Technology, Jinan 250353, PR China. Electronic address: xfx315@bjfu.edu.cn.

PMID: 35240276
DOI: 10.1016/j.biortech.2022.126919

Abstract

The alkali-halophilic Halomonas alkalicola M2 was isolated and developed for an open unsterile polyhydroxyalkanoate (PHA) fermentation from lignocellulose at pH 10.0 and NaCl 70 g/L. The alkaline pretreatment liquid (APL) was converted into PHA by the strain, which was significantly affected by the cultural conditions, including pH, NaCl concentration, nitrogen source, and APL concentration. The extracted PHA was composed of three monomers and similar in physicochemical properties to conventional short chain-length PHA. A record yield of 2.1 and 5.9 g of PHA was accumulated from 100 g dry bamboo powder (BP) by using APL and APL combined with hydrolysate during a 48-h open unsterile fermentation process, respectively. In summary, the alkali-halophilic H. alkalicola M2 achieved the open unsterile fermentation for lignocellulose efficient bioconversion into PHA under high alkalinity and salinity conditions and would be an ideal producer in the field.

Keywords: Alkali-halophilic; Halomonas alkalicola M2; Lignocellulose; Polyhydroxyalkanoate; Unsterile fermentation.

MeSH terms

Alkalies
Halomonas*
Lignin
Polyhydroxyalkanoates*
Sodium Chloride

Substances

Alkalies
Polyhydroxyalkanoates
lignocellulose
Sodium Chloride
Lignin

Supplementary concepts

Halomonas alkalicola