Abstract
SignificanceWhile most small, regulatory RNAs are thought to be "noncoding," a few have been found to also encode a small protein. Here we describe a 164-nucleotide RNA that encodes a 28-amino acid, amphipathic protein, which interacts with aerobic glycerol-3-phosphate dehydrogenase and increases dehydrogenase activity but also base pairs with two mRNAs to reduce expression. The coding and base-pairing sequences overlap, and the two regulatory functions compete.
Keywords:
Hfq; ProQ; catabolite repression; glycerol dehdrogenase; sRNA.
MeSH terms
-
Carbon / metabolism*
-
Culture Media
-
Escherichia coli / growth & development
-
Escherichia coli / metabolism*
-
Escherichia coli Proteins / chemistry
-
Escherichia coli Proteins / metabolism
-
Galactose / metabolism
-
Glycerol / metabolism
-
Glycerolphosphate Dehydrogenase / metabolism
-
Membrane Proteins / chemistry
-
Membrane Proteins / metabolism
-
Protein Biosynthesis
-
RNA, Bacterial / chemistry
-
RNA, Bacterial / metabolism
-
RNA, Bacterial / physiology*
-
RNA, Messenger / metabolism
Substances
-
Culture Media
-
Escherichia coli Proteins
-
Membrane Proteins
-
RNA, Bacterial
-
RNA, Messenger
-
Carbon
-
Glycerolphosphate Dehydrogenase
-
Glycerol
-
Galactose