Monitoring Leukocyte Migration During Atherosclerosis In Vivo

Keiichiro Matoba

doi:10.1007/978-1-0716-1924-7_28

Monitoring Leukocyte Migration During Atherosclerosis In Vivo

Methods Mol Biol. 2022:2419:475-479. doi: 10.1007/978-1-0716-1924-7_28.

Author

Keiichiro Matoba¹

Affiliation

¹ Division of Diabetes, Endocrinology and Metabolism, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo, Japan. matoba@jikei.ac.jp.

PMID: 35237982
DOI: 10.1007/978-1-0716-1924-7_28

Abstract

Transendothelial leukocyte migration is an early event in the progression of vascular inflammation, the underlying molecular mechanism of atherosclerosis. Inflammatory mediators such as adhesion molecules and chemokines are essential in this process. Leukocyte migration into the vascular wall can be monitored by the detection of CD11b-positive immune cells in animal models of atherosclerosis. This chapter will describe an immunohistochemical technique used to evaluate leukocyte migration in vivo.

Keywords: Aorta; CD11b integrin; Immunohistochemistry; Leukocyte migration; Paraffin-embedded tissue.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Atherosclerosis*
Cell Adhesion
Cell Adhesion Molecules
Cell Movement
Chemokines
Inflammation
Leukocytes

Substances

Cell Adhesion Molecules
Chemokines