Electro-analytical sensing of anti-hypotensive agents: application to dosage forms and human urine

Sarah Alharthi; Hany A Batakoushy; Saif A Alharthy; Mahmoud O Abd El-Magied; Waheed M Salem

doi:10.1093/toxres/tfac004

Electro-analytical sensing of anti-hypotensive agents: application to dosage forms and human urine

Toxicol Res (Camb). 2022 Feb 7;11(1):245-254. doi: 10.1093/toxres/tfac004. eCollection 2022 Feb.

Authors

Sarah Alharthi¹, Hany A Batakoushy², Saif A Alharthy³, Mahmoud O Abd El-Magied⁴, Waheed M Salem⁵

Affiliations

¹ Department of Chemistry, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia.
² Pharmaceutical Analysis Department, Faculty of Pharmacy, Menoufia University, Shebin El-Kom, Menoufia 32511, Egypt.
³ Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, P.O. Box 80216, Jeddah 21589, Saudi Arabia.
⁴ Research Sector, Nuclear Materials Authority, P.O. Box 530, El Maadi, Cairo 11936, Egypt.
⁵ Medical Laboratories Department, Faculty of Applied Health Sciences Technology, Menoufia University, Shebin El-Kom, Menoufia 32511, Egypt.

Abstract

A new and valid method was developed for the quantitative voltammetric analysis of midodrine hydrochloride (MID) in pharmaceutical tablets (Midodrine) and biological samples. The method is based on electro-oxidation of MID supported by both disposable pencil electrode (PE) and glassy carbon electrode (GCE). The analysis was carried out using cyclic voltammetry, differential pulse voltammetry (DPV), and square wave voltammetry (SWV) techniques. The proposed analytical method was validated according to ICH guidelines. MID was successively assayed at concentration ranges of 1.15-6.55 and 0.58-3.05 μg mL^-1 at PE. Also, MID was successively assayed at concentration ranges of 1.15-5.28 and 2.86-27.6 μg mL^-1 at GCE for DPV and SWV methods, respectively. The proposed method was successfully used for the analysis of MID in its dosage form and human urine with good recoveries of 99.66 ± 0.33, 99.8 ± 0.45 at PE and 99.8 ± 0.25, 98.7 ± 1.27 at GCE for the DPV and SWV methods, respectively. The suggested method could be applied to the studied drug in the quality control lab as well as in its pharmacokinetic studies.

Keywords: anti-hypotensive agents; disposable pencil electrode; electro-analytical sensing; glassy carbon electrode; human urine; midodrine hydrochloride.