Process optimization and modeling of Cd2+ biosorption onto the free and immobilized Turbinaria ornata using Box-Behnken experimental design

Abstract

The release of effluents containing cadmium ions into aquatic ecosystems is hazardous to humans and marine organisms. In the current investigation, biosorption of Cd²⁺ ions from aqueous solutions by freely suspended and immobilized Turbinaria ornata biomasses was studied. Compared to free cells (94.34%), the maximum Cd²⁺ removal efficiency reached 98.65% for immobilized cells obtained via Box-Behnken design under optimized conditions comprising algal doses of 5.04 g L^-1 and 4.96 g L^-1, pH values of 5.06 and 6.84, and initial cadmium concentrations of 25.2 mg L^-1 and 26.19 mg L^-1, respectively. Langmuir, Freundlich, and Temkin isotherm models were suitably applied, providing the best suit of data for free and immobilized cells, but the Dubinin-Radushkevich model only matched the immobilized algal biomass. The maximum biosorption capacity of Cd²⁺ ions increased with the immobilized cells (29.6 mg g^-1) compared to free cells (23.9 mg g^-1). The Cd²⁺ biosorption data obtained for both biomasses followed pseudo-second-order and Elovich kinetic models. In addition, the biosorption process is controlled by film diffusion followed by intra-particle diffusion. Cd²⁺ biosorption onto the free and immobilized biomasses was spontaneous, feasible, and endothermic in nature, according to the determined thermodynamic parameters. The algal biomass was further examined via SEM/EDX and FTIR before and after Cd²⁺ biosorption. SEM/EDX analysis revealed Cd²⁺ ion binding onto the algal surface. Additionally, FTIR analysis confirmed the presence of numerous functional groups (hydroxyl, carboxyl, amine, phosphate, etc.) participating in Cd²⁺ biosorption. This study verified that immobilized algal biomasses constitute a cost-effective and favorable biosorbent material for heavy metal removal from ecosystems.