Detoxification of ochratoxin A and zearalenone by Pleurotus ostreatus during in vitro gastrointestinal digestion

Clarisse Nobre; Alondra González; Carolina Losoya; José A Teixeira; Ruth Belmares; Luís Abrunhosa

doi:10.1016/j.foodchem.2022.132525

Detoxification of ochratoxin A and zearalenone by Pleurotus ostreatus during in vitro gastrointestinal digestion

Food Chem. 2022 Aug 1:384:132525. doi: 10.1016/j.foodchem.2022.132525. Epub 2022 Feb 22.

Authors

Clarisse Nobre¹, Alondra González², Carolina Losoya³, José A Teixeira⁴, Ruth Belmares³, Luís Abrunhosa⁵

Affiliations

¹ CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal; LABBELS -Associate Laboratory, Braga, Guimarães, Portugal. Electronic address: clarissenobre@gmail.com.
² CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal; LABBELS -Associate Laboratory, Braga, Guimarães, Portugal; Food Research Department, Faculty of Chemistry, Autonomous University of Coahuila, Blvd. V. Carranza and Ing, José Cárdenas s/n Col, República, 25280 Saltillo, Coahuila, Mexico.
³ Food Research Department, Faculty of Chemistry, Autonomous University of Coahuila, Blvd. V. Carranza and Ing, José Cárdenas s/n Col, República, 25280 Saltillo, Coahuila, Mexico.
⁴ CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal; LABBELS -Associate Laboratory, Braga, Guimarães, Portugal.
⁵ CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal; LABBELS -Associate Laboratory, Braga, Guimarães, Portugal. Electronic address: luisjap@deb.uminho.pt.

PMID: 35227998
DOI: 10.1016/j.foodchem.2022.132525

Abstract

Powdered Pleurotusostreatus (PO) was tested for the detoxification of ochratoxin A (OTA) and zearalenone (ZEN) through in vitro gastrointestinal digestion in the absence and presence of ground feed and cornmeal. Ochratoxin α was detected in the chromatograms after OTA elimination, suggesting the presence of an OTA-hydrolytic enzyme in the PO, whereas ZEN was adsorbed by PO. OTA was totally eliminated at pH 5 and 7, while ZEN was better adsorbed at pH 3. In simulated gastrointestinal conditions conducted without feed matrices, PO eliminated OTA by 85% and ZEN by 23% at the end of the intestinal phase. When digestions were conducted with ground feed and cornmeal, PO eliminated OTA by 13 and 34%, and ZEN by 20 and 2%, respectively. PO demonstrated great potential for OTA detoxification, but the feed matrices adsorbed the mycotoxins, reducing their bioaccessibility considerably in the oral and gastric phases and negatively influencing PO detoxification capacity.

Keywords: Biotransformation; In vitro gastrointestinal digestion; Mycotoxins adsorption; Ochratoxin A; Pleurotus ostreatus; Zearalenone.

MeSH terms

Digestion
Food Contamination / analysis
Mycotoxins* / analysis
Ochratoxins* / analysis
Pleurotus*
Zearalenone* / analysis

Substances

Mycotoxins
Ochratoxins
ochratoxin A
Zearalenone