Quantitative and qualitative variability of airborne cyanobacteria and microalgae and their toxins in the coastal zone of the Baltic Sea

Abstract

Apart from viruses and bacteria, cyanobacteria and microalgae present in the atmosphere may pose a threat to the health of humans by inducing illnesses and diseases. Yet, they play an important role in the environment, influencing the Earth's radiation budget by absorbing and scattering solar radiation. The present study determined the daily and seasonal qualitative and qualitative variabilities of airborne cyanobacteria and microalgae during both vegetative and non-vegetative seasons in the coastal zone of the Baltic Sea. Samples were collected from January to December 2020 with a Tisch six-stage microbiological impactor which was used as a substitute for the respiratory tract. The stage levels of the impactor represented the respiratory tract and reproduced lung penetration by airborne particles, which allowed us to assess penetration of cyanobacteria and microalgae to the deepest parts of the human respiratory system. A total of 296 samples of cyanobacteria and microalgae were collected during the day and 276 samples during the night. The results showed that cyanobacteria and microalgae were present in the air all year, and their maximum abundance was 1685 cells m^-3 in July. Furthermore, the ability of these microorganisms to produce the toxin microcystin-LR (MC-LR) was confirmed, which has a high potential negative impact on human health. MC-LR has been found in Nostoc sp., Pseudanabaena sp., Leptolyngbya sp., Synechococcus sp., Gloeocapsa sp., Aphanothece sp., and Rivularia sp. maintained at our Culture Collection of Airborne Algae (CCAA), as well as from air samples. The highest concentrations of MC-LR were recorded in airborne Synechococcus sp. CCAA 46 and amounted to as much as 420 fg cell^-1. In turn, the highest mean concentration of 0.95 μg L^-1 for MC-LR was recorded in an air sample taken in May. This research expands the knowledge on cyanobacteria and microalgae present in the atmosphere in the coastal zone of the southern Baltic Sea. We propose these microorganisms be used as indicators for further research on bioaerosols, which are potentially dangerous to human health.

Keywords: Airborne cyanobacteria; Airborne microalgae; Bioaerosols; Harmful taxa; Microcystin.