The efficiency of RNA-cleaving DNAzymes depends on a large extent on complex formation with their RNA targets. We describe available prediction tools that should help in the design of efficient DNAzymes and show some experimental methods to test the predictions. The main example is for a 10-23 DNAzyme, but the procedure works as well for the 8-17 DNAzyme family.
Keywords: DINAMelt; RNAfold; RNAplfold; poland.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.