Culturable Bacterial Endophytes Associated With Shrubs Growing Along the Draw-Down Zone of Lake Bogoria, Kenya: Assessment of Antifungal Potential Against Fusarium solani and Induction of Bean Root Rot Protection

Priscillar Mumo Mutungi; Vitalis Wafula Wekesa; Justus Onguso; Erustus Kanga; Steve B S Baleba; Hamadi Iddi Boga

doi:10.3389/fpls.2021.796847

Culturable Bacterial Endophytes Associated With Shrubs Growing Along the Draw-Down Zone of Lake Bogoria, Kenya: Assessment of Antifungal Potential Against Fusarium solani and Induction of Bean Root Rot Protection

Front Plant Sci. 2022 Feb 9:12:796847. doi: 10.3389/fpls.2021.796847. eCollection 2021.

Authors

Priscillar Mumo Mutungi^{1

2}, Vitalis Wafula Wekesa³, Justus Onguso¹, Erustus Kanga⁴, Steve B S Baleba⁵, Hamadi Iddi Boga⁶

Affiliations

¹ Institute for Biotechnology Research, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya.
² Ecological Monitoring Department, Kenya Wildlife Service, Nairobi, Kenya.
³ Production Department, Bioline Agrosciences - Dudutech IPM Limited, Nairobi, Kenya.
⁴ State Department for Wildlife, Ministry of Tourism and Wildlife, Nairobi, Kenya.
⁵ Department of Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, Jena, Germany.
⁶ Botany Department, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya.

Abstract

Vascular shrubs growing along the draw-down zones of saline lakes must develop adaptive mechanisms to cope with high salinity, erratic environmental conditions, and other biotic and abiotic stresses. Microbial endophytes from plants growing in these unique environments harbor diverse metabolic and genetic profiles that play an important role in plant growth, health, and survival under stressful conditions. A variety of bacterial endophytes have been isolated from salt tolerant plants but their potential applications in agriculture have not been fully explored. To further address this gap, the present study sought to isolate culturable bacterial endophytes from shrubs growing along the draw-down zone of Lake Bogoria, a saline alkaline lake, and examined their functional characteristics and potential in the biocontrol of the bean root rot pathogen, Fusarium solani. We collected shrubs growing within 5 m distance from the shoreline of Lake Bogoria and isolated 69 bacterial endophytes. The endophytic bacteria were affiliated to three different phyla (Firmicutes, Proteobacteria, and Actinobacteria) with a bias in the genera, Bacillus, and they showed no tissue or plant specificity. All selected isolates were positive for catalase enzyme grown in 1.5 M NaCl; three isolates (B23, B19, and B53) produced indole acetic acid (IAA) and only one isolate, B23 did not solubilize phosphate on Pikovskaya agar. Isolates, B19 and B53 exhibited more than 50% of mycelial inhibition in the dual culture assay and completely inhibited the germination of F. solani spores in co-culture assays while two isolates, B07 and B39 had delayed fungal spore germination after an overnight incubation. All isolates were able to establish endophytic association in the roots, stems, and leaves of been seedlings in both seed soaking and drenching methods. Colonization of bean seedlings by the bacterial endophytes, B19 and B53 resulted in the biocontrol of F. solani in planta, reduced disease severity and incidence, and significantly increased both root and shoot biomass compared to the control. Taxonomic identification using 16S rRNA revealed that the two isolates belong to Enterobacter hormaechei subsp., Xiangfangensis and Bacillus megaterium. Our results demonstrate the potential use of these two isolates in the biocontrol of the bean root rot pathogen, F. solani and plant growth promotion.

Keywords: Bacillus megaterium; Enterobacter hormaechei; Lake Bogoria; biocontrol; endophyte; growth promotion; salinity.