D-allulose ameliorates adiposity through the AMPK-SIRT1-PGC-1α pathway in HFD-induced SD rats

Geum-Hwa Lee; Cheng Peng; Hwa-Young Lee; Seon-Ah Park; The-Hiep Hoang; Jung Hyun Kim; Soonok Sa; Go-Eun Kim; Jung-Sook Han; Han-Jung Chae

doi:10.29219/fnr.v65.7803

D-allulose ameliorates adiposity through the AMPK-SIRT1-PGC-1α pathway in HFD-induced SD rats

Food Nutr Res. 2021 Dec 21:65. doi: 10.29219/fnr.v65.7803. eCollection 2021.

Authors

Geum-Hwa Lee^{1

2}, Cheng Peng¹, Hwa-Young Lee^{1

2}, Seon-Ah Park^{1

2}, The-Hiep Hoang^{1

2}, Jung Hyun Kim³, Soonok Sa⁴, Go-Eun Kim⁴, Jung-Sook Han⁴, Han-Jung Chae^{1

2

5}

Affiliations

¹ Non-Clinical Evaluation Center, Biomedical Research Institute, Jeonbuk National University Hospital, Jeonju, Jeonbuk, Republic of Korea.
² Research Institute of Clinical Medicine of Jeonbuk National University-Biomedical Research Institute of Jeonbuk National University Hospital, Jeonju, Jeonbuk, Republic of Korea.
³ Department of Oral Pathology, School of Dentistry, Jeonbuk National University, Jeonju, Jeonbuk, Republic of Korea.
⁴ Samyang Corp., Bundang-gu, Seongnam-si, Gyeonggi-do, Republic of Korea.
⁵ School of Pharmacy, Jeonbuk National University, Jeonju, Jeonbuk, Republic of Korea.

Abstract

Background: Adiposity is a major health-risk factor, and D-allulose has beneficial effects on adiposity-related metabolic disturbances. However, the modes of action underlying anti-hyperglycemic and hypolipidemic activity are partly understood.

Objective: This study investigated the in vivo and in vitro effects of D-allulose involved in adipogenesis and activation of the AMPK/SIRT1/PGC-1α pathway in high-fat diet (HFD)-fed rats.

Design: In this study, 8-week-old male SD (Sprague Dawley) rats were divided into five groups (n = 8/group), (1) Control (chow diet, 3.5%); (2) 60% HFD; (3) 60% HFD supplemented with allulose powder (AP) at 0.4 g/kg; (4) 60% HFD supplemented with allulose liquid (AL) at 0.4 g/kg; (5) 60% HFD supplemented with glucose (AL) at 0.4 g/kg. All the group received the product through oral gavage for 6 weeks. Control and HFD groups were gavaged with double-distilled water.

Results: Rats receiving AP and AL showed reduced body weight gain and fat accumulation in HFD-fed rats. Also, supplementation of AL/AP regulated the cytokine secretion and recovered biochemical parameters to alleviate metabolic dysfunction and hepatic injury. Additionally, AL/AP administration improved adipocyte differentiation via regulation of the PPARγ and C/EBPα signaling pathway and adipogenesis-related genes owing to the combined effect of the AMPK/SIRT1 pathway. Furthermore, AL/AP treatment mediated PGC-1α expression triggering mitochondrial genesis via activating the AMPK phosphorylation and SIRT1 deacetylation activity in adipose tissue.

Conclusion: The anti-adiposity activity of D-allulose is observed on a marked alleviation in adipogenesis and AMPK/SIRT1/PGC-1α deacetylation in the adipose tissue of HFD-fed rat.

Keywords: AMPK; D-allulose; SIRT1; adipose tissue; adiposity.