Hyaluronic acid and human/bovine serum albumin shelled nanocapsules: Interaction with mucins and in vitro digestibility of interfacial films

Teresa Del Castillo-Santaella; Aixa Aguilera-Garrido; Francisco Galisteo-González; María José Gálvez-Ruiz; José Antonio Molina-Bolívar; Julia Maldonado-Valderrama

doi:10.1016/j.foodchem.2022.132330

Hyaluronic acid and human/bovine serum albumin shelled nanocapsules: Interaction with mucins and in vitro digestibility of interfacial films

Food Chem. 2022 Jul 30:383:132330. doi: 10.1016/j.foodchem.2022.132330. Epub 2022 Feb 3.

Authors

Teresa Del Castillo-Santaella¹, Aixa Aguilera-Garrido², Francisco Galisteo-González², María José Gálvez-Ruiz³, José Antonio Molina-Bolívar⁴, Julia Maldonado-Valderrama⁵

Affiliations

¹ Department of Applied Physics, University of Granada, Avenida de Fuente Nueva, s/n, C.P. 18071 Granada, Spain; Department of Physical Chemistry, University of Granada, Campus Universitario s/n, C.P. 1807 Granada, Spain.
² Department of Applied Physics, University of Granada, Avenida de Fuente Nueva, s/n, C.P. 18071 Granada, Spain.
³ Department of Applied Physics, University of Granada, Avenida de Fuente Nueva, s/n, C.P. 18071 Granada, Spain; Excellence Research Unit "Modeling Nature" (MNat), University of Granada, Avda. del Hospicio, s/n, C.P. 18010 Granada, Spain.
⁴ Excellence Research Unit "Modeling Nature" (MNat), University of Granada, Avda. del Hospicio, s/n, C.P. 18010 Granada, Spain; Department of Applied Physics II, Engineering School, University of Málaga, 29071 Málaga, Spain.
⁵ Department of Applied Physics, University of Granada, Avenida de Fuente Nueva, s/n, C.P. 18071 Granada, Spain; Excellence Research Unit "Modeling Nature" (MNat), University of Granada, Avda. del Hospicio, s/n, C.P. 18010 Granada, Spain. Electronic address: julia@ugr.es.

PMID: 35219153
DOI: 10.1016/j.foodchem.2022.132330

Abstract

Liquid lipid nanocapsules are oil droplets surrounded by a protective shell, which enable high load and allow controlled delivery of lipophilic compounds. However, their use in food formulations requires analysing their digestibility and interaction with mucin. Here, serum albumins and hyaluronic acid shelled olive oil nanocapsules are analysed to discern differences between human and bovine variants, the latter usually used as model system. Interfacial interaction of albumins and hyaluronic acid reveals that human albumin presents limited conformational changes upon adsorption, which increase by complexation with the polysaccharide present at the interface. The latter also promotes hydrophobic interactions with mucin, especially at pH 3 and protects albumin interfacial layer under in vitro gastric digestion. The interfacial unfolding induced in human albumin by hyaluronic acid facilitates in vitro lipolysis while its limited conformational changes provide the largest protection against in vitro lipolysis.

Keywords: Albumin; Digestion; Emulsion; Hyaluronic acid; Interfacial tension; Mucin.

MeSH terms

Emulsions / chemistry
Humans
Hyaluronic Acid
Mucins
Nanocapsules* / chemistry
Serum Albumin, Bovine
Serum Albumin, Human

Substances

Emulsions
Mucins
Nanocapsules
Serum Albumin, Bovine
Hyaluronic Acid
Serum Albumin, Human