Preparation and evaluation of two silica-based hydrophilic-hydrophobic and acid-base balanced stationary phases via in-situ surface polymerization

Chao Fan; Jia Chen; Hui Li; Kaijun Quan; Hongdeng Qiu

doi:10.1016/j.chroma.2022.462912

Preparation and evaluation of two silica-based hydrophilic-hydrophobic and acid-base balanced stationary phases via in-situ surface polymerization

J Chromatogr A. 2022 Mar 29:1667:462912. doi: 10.1016/j.chroma.2022.462912. Epub 2022 Feb 17.

Authors

Chao Fan¹, Jia Chen², Hui Li³, Kaijun Quan³, Hongdeng Qiu⁴

Affiliations

¹ CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China; University of Chinese Academy of Sciences, Beijing 100049, China.
² CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China. Electronic address: jiachen@licp.cas.cn.
³ CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China.
⁴ CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China; University of Chinese Academy of Sciences, Beijing 100049, China; College of Chemistry, Zhengzhou University, Zhengzhou 450001, China. Electronic address: hdqiu@licp.cas.cn.

PMID: 35219109
DOI: 10.1016/j.chroma.2022.462912

Abstract

Two new hydrophilic-hydrophobic and acid-base balanced liquid chromatographic stationary phases were obtained by using two long-chain monomers including undecylenic acid (UA) and oleic acid (OA) combined with short-chain 4-vinyl pyridine (Py) in-situ polymerized on silica microspheres surfaces, respectively. These two stationary phases can be used for reversed-phase liquid chromatography (RPLC) and hydrophilic interaction liquid chromatography (HILIC) modes. Hydrophilic, hydrophobic, acidic and basic analytes can be separated on two new stationary phases in RPLC or HILIC mode. Compared with commercial hydrophobic C18 columns and other HILIC columns, two new stationary phases have excellent selectivity and separation performance in the separation of 9 polycyclic aromatic hydrocarbons, 10 aromatic acids, 10 anilines and 5 oxazolidinones, respectively. This novel polymer-functionalized silica design strategy has opened up new ideas for the future of mixed-mode stationary phases with multiple functions and specificity.

Keywords: Acid-base balance; HPLC; Hydrophilic-hydrophobic; Mixed-mode; Stationary phase.

MeSH terms

Chromatography, Reverse-Phase* / methods
Hydrophobic and Hydrophilic Interactions
Microspheres
Polymerization
Silicon Dioxide* / chemistry

Substances

Silicon Dioxide