Establishment of an inducible cell line for Hepatitis B virus genotype C2 and its pharmacological responses to interferons

Shaodong Jiang; Xin Wang; Kaili Chen; Pengyuan Yang

doi:10.1016/j.phrs.2022.106142

Establishment of an inducible cell line for Hepatitis B virus genotype C2 and its pharmacological responses to interferons

Pharmacol Res. 2022 Apr:178:106142. doi: 10.1016/j.phrs.2022.106142. Epub 2022 Feb 24.

Authors

Shaodong Jiang¹, Xin Wang², Kaili Chen¹, Pengyuan Yang³

Affiliations

¹ CAS Key Laboratory of Infection and Immunity, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China.
² CAS Key Laboratory of Infection and Immunity, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
³ CAS Key Laboratory of Infection and Immunity, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: pyyang@ibp.ac.cn.

PMID: 35218895
DOI: 10.1016/j.phrs.2022.106142

Abstract

Hepatitis B virus (HBV) genotype C is closely associated with poor prognosis, contributing greatly to heavy chronic hepatitis B (CHB)-related liver disease burden in China and worldwide. However, the mechanistic studies on genotype C of HBV remain largely limited, partially because of a long-term lack of genotype C HBV-based stable cellular tools. According to a bioinformatics analysis on the sub-genotype C2 HBV that is predominantly endemic in China, we selected 17.3 strain as a representative isolate. With a Tet-off gene expression system, an inducible viral replication and virion DNA production of genotype C2 HBV were achieved in a cell line carrying persistent rcDNA-cccDNA recycling, termed HepG2-17.3, can be useful for virological studies. Additionally, this cell line has been formatted into cell-based assay that permits particular pharmacological screening of drug candidates, such as interferon regimens, for evaluations of the inhibitory effects on genotype C2 HBV replication.

Keywords: Chronic hepatitis B (CHB); Genotype C2 HBV; Inducible replication cells; Interferon.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line
DNA, Viral / genetics
DNA, Viral / metabolism
Genotype
Hepatitis B virus* / genetics
Interferons* / metabolism

Substances

DNA, Viral
Interferons