Nanometer-Scale Molecular Mapping by Super-resolution Fluorescence Microscopy

Vito Mennella; Zhen Liu

doi:10.1007/978-1-0716-2051-9_18

Nanometer-Scale Molecular Mapping by Super-resolution Fluorescence Microscopy

Methods Mol Biol. 2022:2440:305-326. doi: 10.1007/978-1-0716-2051-9_18.

Authors

Vito Mennella¹, Zhen Liu²

Affiliations

¹ MRC Toxicology Unit, School of Biological Sciences, University of Cambridge, Cambridge, UK. vm430@cam.ac.uk.
² Division of Life Science, The Hong Kong University of Science and Technology, Hong Kong SAR, China.

PMID: 35218547
DOI: 10.1007/978-1-0716-2051-9_18

Abstract

The structural organization of macromolecules and their association in assemblies and organelles is key to understand cellular function. Super-resolution fluorescence microscopy has expanded our toolbox for examining such nanometer-scale cellular structures, by enabling positional mapping of proteins in situ. Here, we detail the workflow to build nanometer-scale maps focusing on two complementary super-resolution modalities: structured illumination microscopy (SIM) and stochastic optical reconstruction microscopy (STORM).

Keywords: Centrosome; Cilia; Macromolecular assembly organization; SIM; STORM; Subdiffraction imaging; Super-resolution fluorescence microscopy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Macromolecular Substances
Microscopy, Fluorescence
Organelles*

Substances

Macromolecular Substances

Grants and funding

MC_UU_00025/13/MRC_/Medical Research Council/United Kingdom