Bimolecular Fluorescence Complementation to Visualize Protein-Protein Interactions in Cells

Cassandra R Edgar; Jimmy D Dikeakos

doi:10.1007/978-1-0716-2051-9_5

Bimolecular Fluorescence Complementation to Visualize Protein-Protein Interactions in Cells

Methods Mol Biol. 2022:2440:91-97. doi: 10.1007/978-1-0716-2051-9_5.

Authors

Cassandra R Edgar¹, Jimmy D Dikeakos²

Affiliations

¹ Department of Microbiology and Immunology, The University of Western Ontario, London, ON, Canada.
² Department of Microbiology and Immunology, The University of Western Ontario, London, ON, Canada. jimmy.dikeakos@uwo.ca.

PMID: 35218534
DOI: 10.1007/978-1-0716-2051-9_5

Abstract

Examining protein-protein interactions provides critical insight into numerous human diseases and infections. Here we describe a protocol for bimolecular fluorescence complementation, which can be used to directly visualize and characterize intracellular protein-protein interactions and ascertain their localization using fluorescence microscopy.

Keywords: Bimolecular fluorescence complementation; Microscopy; Protein–protein interactions; Subcellular localization.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Fluorescence
Humans
Luminescent Proteins / genetics
Microscopy, Fluorescence / methods
Protein Interaction Mapping* / methods

Substances

Luminescent Proteins

Grants and funding

389413/CIHR/Canada