Thymic stromal lymphopoietin controls hair growth

Jessica L Shannon; David L Corcoran; John C Murray; Steven F Ziegler; Amanda S MacLeod; Jennifer Y Zhang

doi:10.1016/j.stemcr.2022.01.017

Thymic stromal lymphopoietin controls hair growth

Stem Cell Reports. 2022 Mar 8;17(3):649-663. doi: 10.1016/j.stemcr.2022.01.017. Epub 2022 Feb 24.

Authors

Jessica L Shannon¹, David L Corcoran², John C Murray³, Steven F Ziegler⁴, Amanda S MacLeod⁵, Jennifer Y Zhang⁶

Affiliations

¹ Department of Dermatology, Duke University, P.O. Box 103052, Durham, NC 27710, USA; Department of Immunology, Duke University, Durham, NC 27710, USA.
² Genomic and Computational Biology, Duke University, Durham, NC 27705, USA.
³ Department of Dermatology, Duke University, P.O. Box 103052, Durham, NC 27710, USA.
⁴ Benaroya Research Institute, Seattle, WA 98101, USA; Department of Immunology, University of Washington, Seattle, WA 98195, USA.
⁵ Department of Dermatology, Duke University, P.O. Box 103052, Durham, NC 27710, USA; Department of Immunology, Duke University, Durham, NC 27710, USA; Molecular Genetics and Microbiology, Duke University, Durham, NC 27710, USA.
⁶ Department of Dermatology, Duke University, P.O. Box 103052, Durham, NC 27710, USA; Department of Pathology, Duke University Medical Center, Durham, NC 27710, USA. Electronic address: jennifer.zhang@duke.edu.

Abstract

Skin tissue regeneration after injury involves the production and integration of signals by stem cells residing in hair follicles (HFSCs). Much remains unknown about how specific wound-derived factors modulate stem cell contribution to hair growth. We demonstrate that thymic stromal lymphopoietin (TSLP) is produced in response to skin injury and during the anagen phase of the hair cycle. Intradermal injection of TSLP promoted wound-induced hair growth (WIHG), whereas neutralizing TSLP receptor (TSLPR) inhibited WIHG. Using flow cytometry and fluorescent immunostaining, we found that TSLP promoted proliferation of transit-amplifying cells. Lgr5^CreER-mediated deletion of Tslpr in HFSCs inhibited both wound-induced and exogenous TSLP-induced hair growth. Our data highlight a novel function for TSLP in regulation of hair follicle activity during homeostasis and wound healing.

Keywords: TSLP; hair follicle; hair follicle stem cell; human; immunology; mouse; regeneration; tissue stem cell.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Cytokines* / metabolism
Hair / metabolism
Receptors, Cytokine* / genetics
Receptors, Cytokine* / metabolism
Skin / metabolism
Thymic Stromal Lymphopoietin

Substances

Cytokines
Receptors, Cytokine
Thymic Stromal Lymphopoietin

Abstract

Publication types

MeSH terms

Substances

Grants and funding