Isolation of the Stromal Vascular Fraction from Adipose Tissue and Subsequent Differentiation into White or Beige Adipocytes

Jared S Farrar; Rebecca K Martin

doi:10.1007/978-1-0716-2128-8_10

Isolation of the Stromal Vascular Fraction from Adipose Tissue and Subsequent Differentiation into White or Beige Adipocytes

Methods Mol Biol. 2022:2455:103-115. doi: 10.1007/978-1-0716-2128-8_10.

Authors

Jared S Farrar¹, Rebecca K Martin²

Affiliations

¹ Center for Clinical and Translational Research, Virginia Commonwealth University, Richmond, VA, USA.
² Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA, USA. Rebecca.Martin@vcuhealth.org.

Abstract

Non-alcoholic steatohepatitis (NASH) is linked to adipose tissue dysfunction, with weight loss being the only treatment shown to reverse it. Due to this correlation with obesity, the study of adipose tissue and adipocytes is an important step in understanding the pathogenesis of this disease. Here, we describe the isolation process of the stromal vascular fraction (SVF) of adipose tissue. The SVF contains the foundational cells that will differentiate into adipocytes. These cells can be isolated and subsequently differentiated in vitro into white and beige adipocytes. We outline the in vitro differentiation of pre-adipocytes into cultured white and beige adipocytes using both human and mouse adipose tissue.

Keywords: Adipose tissue; Beige adipocytes; Browning; Stromal vascular fraction; White adipocytes.

Publication types

Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural

MeSH terms

Adipocytes
Adipocytes, Beige*
Adipocytes, White
Adipose Tissue
Animals
Cell Differentiation
Mice
Stromal Vascular Fraction

Grants and funding

UL1 TR002649/TR/NCATS NIH HHS/United States