Generation of a Diet-Induced Mouse Model of Nonalcoholic Fatty Liver Disease

Amon Asgharpour; Arun J Sanyal

doi:10.1007/978-1-0716-2128-8_2

Generation of a Diet-Induced Mouse Model of Nonalcoholic Fatty Liver Disease

Methods Mol Biol. 2022:2455:19-30. doi: 10.1007/978-1-0716-2128-8_2.

Authors

Amon Asgharpour¹, Arun J Sanyal²

Affiliations

¹ Division of Gastroenterology, Hepatology and Nutrition, Department of Internal Medicine, Virginia Commonwealth University, Richmond, VA, USA. amon.asgharpour@vcuhealth.org.
² Division of Gastroenterology, Hepatology and Nutrition, Department of Internal Medicine, Virginia Commonwealth University, Richmond, VA, USA.

PMID: 35212982
DOI: 10.1007/978-1-0716-2128-8_2

Abstract

The obesity epidemic is driving the increased prevalence of nonalcoholic fatty liver disease (NAFLD) globally. The more aggressive subtype of NAFLD, nonalcoholic steatohepatitis (NASH), can lead to progressive disease and ultimately lead to cirrhosis, liver cancer, and death. There are many unmet needs in the field of NAFLD including understanding of molecular mechanisms driving disease, natural history, risk for liver cancer, and most importantly FDA approved therapeutics. Animal models serve as a tool to aid in answering some of these questions. Here, we describe the diet-induced animal model of NAFLD (DIAMOND), a mouse model with many characteristics that mimic human NASH.

Keywords: DIAMOND; Hepatocellular carcinoma (HCC ); Liver fibrosis; Mouse model; Nonalcoholic fatty liver disease (NAFLD); Nonalcoholic steatohepatitis (NASH ).

MeSH terms

Animals
Diet / adverse effects
Disease Models, Animal
Liver / pathology
Liver Cirrhosis / pathology
Liver Neoplasms* / pathology
Mice
Non-alcoholic Fatty Liver Disease* / etiology
Non-alcoholic Fatty Liver Disease* / pathology