Analysis of Activity and Expression of the NLRP3, AIM2, and NLRC4 Inflammasome in Whole Blood

Lev Grinstein; Stefan Winkler

doi:10.1007/978-1-0716-2144-8_11

Analysis of Activity and Expression of the NLRP3, AIM2, and NLRC4 Inflammasome in Whole Blood

Methods Mol Biol. 2022:2459:105-116. doi: 10.1007/978-1-0716-2144-8_11.

Authors

Lev Grinstein¹, Stefan Winkler²

Affiliations

¹ Department of Pediatrics, University Medical Centre Hamburg-Eppendorf, Hamburg, Germany.
² Department of Pediatrics, University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany. stefan.winkler@uniklinikum-dresden.de.

PMID: 35212959
DOI: 10.1007/978-1-0716-2144-8_11

Abstract

Pro-inflammatory caspase-1 is a key player in innate immunity. Following activation in heterogenic protein complexes called the inflammasome , caspase-1 processes IL-1β and IL-18 to their mature forms and triggers pyroptosis. Here, we describe a small-volume whole blood assay facilitating the measurement of caspase-1 activity and inflammasome-related gene expression following specific stimulation of either the NLRP3, NLRC4, or AIM2 inflammasome .

Keywords: AIM2; Caspase-1; Inflammasome; MCC950; NLRC4; NLRP3; Whole blood assay.

MeSH terms

Caspase 1 / metabolism
DNA-Binding Proteins / metabolism
Inflammasomes* / metabolism
Interleukin-1beta / metabolism
NLR Family, Pyrin Domain-Containing 3 Protein* / metabolism
Pyroptosis

Substances

DNA-Binding Proteins
Inflammasomes
Interleukin-1beta
NLR Family, Pyrin Domain-Containing 3 Protein
Caspase 1