Anti-Diabetic Indole-Terpenoids From Penicillium sp. HFF16 Isolated From the Rhizosphere Soil of Cynanchum bungei Decne

Na Xiao; Yiru Xu; Xinru Zhang; Haonan Li; Shengnan Zhang; Ang Xiao; Jinyi Yu; Mingtian Yang; Fujin Lv; Mingyu Zhang; Gangping Hao; Guotong Chen; Liman Zhou; Fandong Kong; Guojun Pan

doi:10.3389/fchem.2021.792810

Anti-Diabetic Indole-Terpenoids From Penicillium sp. HFF16 Isolated From the Rhizosphere Soil of Cynanchum bungei Decne

Front Chem. 2022 Feb 8:9:792810. doi: 10.3389/fchem.2021.792810. eCollection 2021.

Authors

Na Xiao¹, Yiru Xu², Xinru Zhang², Haonan Li², Shengnan Zhang², Ang Xiao², Jinyi Yu², Mingtian Yang², Fujin Lv², Mingyu Zhang², Gangping Hao², Guotong Chen³, Liman Zhou³, Fandong Kong³, Guojun Pan²

Affiliations

¹ State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agriculture University, Tai'an, China.
² College of Life Sciences, Shandong First Medical University, Shandong Academy of Medical Sciences, Tai'an, China.
³ Key Laboratory of Chemistry and Engineering of Forest Products, State Ethnic Affairs Commission, Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, Guangxi Collaborative Innovation Center for Chemistry and Engineering of Forest Products, School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning, China.

Abstract

Finding novel anti-diabetic compounds with effective suppression activities against hepatic glucagon response is urgently required for the development of new drugs against diabetes. Fungi are well known for their ability to produce new bioactive secondary metabolites. As part of our ongoing research, five new indole-terpenoids (1-5), named encindolenes D-H, were isolated from the fungus Penicillium sp. HFF16 from the rhizosphere soil of Cynanchum bungei Decne. The structures of the compounds were elucidated by spectroscopic data and ECD analysis. In the anti-diabetic activity assay, compounds 1-5 could inhibit the hepatic glucose production with EC₅₀ values of 17.6, 30.1, 21.3, 9.6, and 9.9 μM, respectively, and decrease the cAMP contents in glucagon-induced HepG2 cells.

Keywords: Cynanchum bungei Decne; Penicillium sp. HFF16; anti- diabetic activity; fungus; indole-terpenoids.