Solea senegalensis Bacterial Intestinal Microbiota Is Affected by Low Dietary Inclusion of Ulva ohnoi

Isabel M Cerezo; Milena Fumanal; Silvana T Tapia-Paniagua; Rocio Bautista; Victoria Anguís; Catalina Fernández-Díaz; Francisco Javier Alarcón; Miguel A Moriñigo; M Carmen Balebona

doi:10.3389/fmicb.2021.801744

Solea senegalensis Bacterial Intestinal Microbiota Is Affected by Low Dietary Inclusion of Ulva ohnoi

Front Microbiol. 2022 Feb 8:12:801744. doi: 10.3389/fmicb.2021.801744. eCollection 2021.

Authors

Isabel M Cerezo^{1

2}, Milena Fumanal¹, Silvana T Tapia-Paniagua¹, Rocio Bautista², Victoria Anguís³, Catalina Fernández-Díaz³, Francisco Javier Alarcón⁴, Miguel A Moriñigo¹, M Carmen Balebona¹

Affiliations

¹ Departamento de Microbiología, Facultad de Ciencias, Ceimar-Universidad de Málaga, Málaga, Spain.
² Unidad de Bioinformática - SCBI, Universidad de Málaga, Málaga, Spain.
³ IFAPA Centro El Toruño, El Puerto de Santa María, Spain.
⁴ Departamento de Biología y Geología, Ceimar-Universidad de Almería, Almería, Spain.

Abstract

The inclusion of macroalgae in the diets of farmed fish offers the opportunity for an added-value dietary ingredient to the nutraceutical feed. The composition of algae varies greatly among species. Several Ulva species have been considered in aquafeed formulations for different farmed fish, and Ulva ohnoi is being applied recently. However, the effects of seaweed dietary inclusion on the host must be evaluated. Considering the important role of the host intestinal microbiota, the potential effects of U. ohnoi dietary inclusion need to be studied. In this study, the characterization of the intestinal microbiome of Solea senegalensis, a flatfish with high potential for aquaculture in South Europe, receiving U. ohnoi (5%)-supplemented diet for 90 days has been carried out. In addition, the functional profiles of bacterial communities have been determined by using PICRUSt, a computational approach to predict the functional composition of a metagenome by using marker gene data and a database of reference genomes. The results show that long-term dietary administration of U. ohnoi (5%)-supplemented feed modulates S. senegalensis intestinal microbiota, especially in the posterior intestinal section. Increased relative abundance of Vibrio jointly with decreased Stenotrophomonas genus has been detected in fish receiving Ulva diet compared to control-fed fish. The influence of the diet on the intestinal functionality of S. senegalensis has been studied for the first time. Changes in bacterial composition were accompanied by differences in predicted microbiota functionality. Increased abundance of predicted genes involved in xenobiotic biodegradation and metabolism were observed in the microbiota when U. ohnoi diet was used. On the contrary, predicted percentages of genes associated to penicillin and cephalosporin biosynthesis as well as beta-lactam resistance were reduced after feeding with Ulva diet.

Keywords: Solea senegalensis; Ulva; algae; aquafeed; microbiome.