Mechanical stress effects on transcriptional regulation of genes encoding microtubule- and actin-associated proteins

Galina V Shevchenko; Konstantin V Krutovsky

doi:10.1007/s12298-021-01123-x

Mechanical stress effects on transcriptional regulation of genes encoding microtubule- and actin-associated proteins

Physiol Mol Biol Plants. 2022 Jan;28(1):17-30. doi: 10.1007/s12298-021-01123-x. Epub 2022 Jan 21.

Authors

Galina V Shevchenko¹, Konstantin V Krutovsky^{2

3

4

5

6}

Affiliations

¹ Institute of Botany, National Academy of Sciences of Ukraine, Kiev, 01004 Ukraine.
² Department of Forest Genetics and Forest Tree Breeding, Georg-August University of Göttingen, Büsgenweg 2, 37077 Göttingen, Germany.
³ Center for Integrated Breeding Research, Georg-August University of Göttingen, 37075 Göttingen, Germany.
⁴ Laboratory of Population Genetics, N.I. Vavilov Institute of General Genetics, Russian Academy of Sciences, 119333 Moscow, Russian Federation.
⁵ Department of Genomics and Bioinformatics, Laboratory of Forest Genomics, Genome Research and Education Center, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, 660036 Krasnoyarsk, Russian Federation.
⁶ Scientific and Methodological Center, G. F. Morozov Voronezh State University of Forestry and Technologies, 394087 Voronezh, Russian Federation.

Abstract

Plant cytoskeleton regulation has been studied using a new approach based on both (1) pharmacological analysis of tubulin and actin inhibitors and (2) mechanical stimulation achieved by using a slow-rotating (2 rpm) clinostat in combination with transcriptional analysis of genes encoding TUA6, ACT2, MAP65-1, CLASP, PLDδ, FH4 and FH1 proteins in Arabidopsis thaliana seedling roots. The obtained data suggest feedback between the organization of microtubule (MT) and actin filament (AF) networks and the expression of the ACT2, TUA6, MAP65-1, CLASP and FH1/FH4 genes. Different regulation of feedback between MT/AF organization and TUA6, ACT2, MAP65-1, CLASP, FH4 and FH1 gene expression was noted during slow clinorotation, possibly due to altered mechanical impact on the cortical cytoskeleton. For the first time, the expression of the tubulin-associated gene MAP65-1 was shown to be dependent upon the organization of AFs. TUA6, MAP65-1, CLASP, FH1 and FH4 likely participate in mechanical signal transduction. Our work demonstrated that slow clinorotation is able to cause mechanical stress.

Keywords: Actin filaments; Arabidopsis; Genes encoding tubulin- and actin-associated proteins; Mechanical stress; Microtubules; Slow rotating clinostats.