This Feature Issue of Optics Express is organized in conjunction with the 2021 Optica (OSA) conference on 3D Image Acquisition and Display: Technology, Perception and Applications which was held virtually from 19 to 23, July 2021 as part of the Imaging and Sensing Congress 2021. This Feature Issue presents 29 articles which cover the topics and scope of the 2021 3D conference. This Introduction provides a summary of these articles.