Mitochondrial Genomes Yield Insights into the Basal Lineages of Ichneumonid Wasps (Hymenoptera: Ichneumonidae)

Boying Zheng; Yuanyuan Han; Ruizhong Yuan; Jingxian Liu; Pu Tang; Cornelis van Achterberg; Xuexin Chen

doi:10.3390/genes13020218

Mitochondrial Genomes Yield Insights into the Basal Lineages of Ichneumonid Wasps (Hymenoptera: Ichneumonidae)

Genes (Basel). 2022 Jan 25;13(2):218. doi: 10.3390/genes13020218.

Authors

Boying Zheng^{1

2}, Yuanyuan Han^{1

2}, Ruizhong Yuan^{1

2}, Jingxian Liu³, Pu Tang^{1

2

4

5}, Cornelis van Achterberg^{1

2}, Xuexin Chen^{1

2

4

5}

Affiliations

¹ State Key Laboratory of Rice Biology, Zhejiang University, Hangzhou 310058, China.
² Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China.
³ Department of Entomology, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China.
⁴ Zhejiang Provincial Key Laboratory of Biology of Crop Pathogens and Insects, Zhejiang University, Hangzhou 310058, China.
⁵ Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Zhejiang University, Hangzhou 310058, China.

Abstract

We obtained four mitochondrial genomes of Odontocolon sp., Xorides funiuensis, Euceros kiushuensis and Euceros serricornis, which represent the first two representatives from subfamily Eucerotinae and Xoridinae (Ichneumonidae), respectively. All of the 4 newly sequenced mitochondrial genomes contain 13 protein-coding genes (PCGs) and most 24 RNA genes. Furthermore, they all have novel tRNA rearrangement patterns comparing with published mitogenomes in Ichneumonidae. For the tRNA cluster trnI-trnQ-trnM, X. funiuensis is shuffled as trnM-trnI-trnQ with trnQ inversed, while Odontocolon sp. with a remote translocation of trnK, shuffling as trnI-trnM-trnQ. E. kiushuensis and E. serricornis shared the same cluster trnQ-trnY-trnW-trnC. Finally, we reconstructed the phylogenetic relationships among the sequenced subfamilies of Ichneumonidae based on nucleotides and amino acids sequences of 13 PCGs in mitochondrial genomes, and the results of both the maximum likelihood and Bayesian inference analyses highly support that Eucerotinae is the basal ichneumonid lineage rather than Xoridinae.

Keywords: Eucerotinae; Xoridinae; gene rearrangement; ichneumonid wasps; mitochondrial genome; phylogeny.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bayes Theorem
Genome, Mitochondrial*
Phylogeny
RNA, Transfer / genetics
Sequence Analysis, DNA / methods
Wasps* / genetics

Substances

RNA, Transfer