RNA-Seq Technology Reveals the Mechanism of SDT Combined With Novel Nanobubbles Against HCC

Haitao Shang; Yichi Chen; Chunyue Wang; Shentao Zhang; Bolin Wu; Xitian Liang; Zhao Liu; Qiucheng Wang; Wen Cheng

doi:10.3389/fmolb.2021.791331

RNA-Seq Technology Reveals the Mechanism of SDT Combined With Novel Nanobubbles Against HCC

Front Mol Biosci. 2022 Feb 7:8:791331. doi: 10.3389/fmolb.2021.791331. eCollection 2021.

Authors

Haitao Shang¹, Yichi Chen¹, Chunyue Wang¹, Shentao Zhang¹, Bolin Wu^{1

2}, Xitian Liang¹, Zhao Liu¹, Qiucheng Wang¹, Wen Cheng^{1

2}

Affiliations

¹ Department of Ultrasound, Harbin Medical University Cancer Hospital, Harbin, China.
² Department of Interventional Ultrasound, Harbin Medical University Cancer Hospital, Harbin, China.

Abstract

Sonodynamic therapy is widely used in the treatment and research of hepatocellular carcinoma. A novel targeted nanobubble complex mediated with Hematoporphyrin monomethyl ether and Lonidamine was structured as a sensitizer, characterized the properties, and studied the therapeutic effect on hepatocellular carcinoma. The complexes can promote the apoptosis of hepatocellular carcinoma cells and work better in combination with sonodynamic therapy. The differential expression of multiple types of RNA in hepatocellular carcinoma with sonodynamic therapy can be identified accurately with high-throughput RNA sequencing. The differential expressions of mRNA, lncRNA, and circRNA were analyzed by RNA-Seq. The enrichment analyses (Gene Ontology and KEGG) prompted the meaningful genes and pathways in the process of sonodynamic therapy in hepatocellular carcinoma cells. HMME-LND@C₃F₈-NBs conjugated with ultrasound is confirmed efficiently for inhibiting the development of hepatocellular carcinoma cells, and it is a combination of multiple genes and mechanisms.

Keywords: HMME; LND; RNA-seq; hepatocellular carcinoma; nanobubble; sonodynamic therapy.