Pharmacological properties and preliminary phytochemical analysis of Pseudocaryopteris foetida (D.Don) P.D. Cantino leaves

Tayyiba Afzal; Yamin Bibi; Muhammad Ishaque; Saadia Masood; Abdul Qayyum; Sobia Nisa; Zahid Hussain Shah; Hameed Alsamadany; Gyuhwa Chung

doi:10.1016/j.sjbs.2021.09.048

Pharmacological properties and preliminary phytochemical analysis of Pseudocaryopteris foetida (D.Don) P.D. Cantino leaves

Saudi J Biol Sci. 2022 Feb;29(2):1185-1190. doi: 10.1016/j.sjbs.2021.09.048. Epub 2021 Sep 20.

Authors

Tayyiba Afzal¹, Yamin Bibi¹, Muhammad Ishaque¹, Saadia Masood², Abdul Qayyum³, Sobia Nisa⁴, Zahid Hussain Shah⁵, Hameed Alsamadany⁶, Gyuhwa Chung⁷

Affiliations

¹ Department of Botany, PMAS-Arid Agriculture University Rawalpindi, Rawalpindi 46300 Pakistan.
² Department of Statistics & Mathematics, PMAS-Arid Agriculture University Rawalpindi, Rawalpindi 46300, Pakistan.
³ Department of Agronomy, The University of Haripur, Haripur 22620, Pakistan.
⁴ Department of Microbiology, The University of Haripur, Haripur 22620, Pakistan.
⁵ Department of Plant Breeding and Genetics, PMAS-Arid Agriculture University Rawalpindi, Rawalpindi 46300, Pakistan.
⁶ Department of Biological Sciences, King Abdul Aziz University, Jeddah, Saudi Arabia.
⁷ Department of Biotechnology, Chonnam National University, Yeosu, Chonnam 59626, South Korea.

Abstract

Medicinal plants have significant contribution in pharmaceutical industries being producers of compounds utilized as precursors for drug development. A plant of Lamiaceae family; Pseudocaryopteris foetida had not been investigated for its biomedical potential. Current research was aimed to investigate phytochemical analysis, cytotoxic potential and antioxidant activity of crude methanolic extract and fractions of Pseudocaryopteris foetida (leaves). The preliminary phytochemical analysis of crude methanolic extracts and fractions of Pseudocaryopteris foetida revealed that plant is rich in phenolic and flavonoid classes of secondary metabolites while presence of tannin was observed only in crude methanolic extract. The cytotoxicity was determined using brine shrimp lethality test. Different concentrations (25, 50, 100, 150, 200 and 250 µg/mL) of crude methanolic extract and fractions exhibited dose dependent cytotoxicity. However, The LD₅₀ for all the extracts was more than 200 µg/mL indicating weak cytotoxic potential of Pseudocaryopteris foetida. The antioxidant capabilities of crude methanolic extract and fraction of Pseudocaryopteris foetida were analyzed by in vitro bio assays including DPPH, ABTS, Reducing power and phosphomolybdate antioxidant assays using ascorbic acid as standard. The crude methanolic extract showed IC₅₀ (256.38 ± 0.6 and 314.95 ± 1.1 µg/mL) for DPPH and ABTS respectively, while total antioxidant capacity was calculated as 55.79 ± 0.5 µg/mL for crude methanolic extract of Pseudocaryopteris foetida while ascorbic acid indicated total antioxidant capacity of 71.89 ± 2.3 µg/mL. Study concluded that leaves of Pseudocaryopteris foetida were the rich source of antioxidant phytochemicals. Based on preliminary investigations further research should be focused to isolate bioactive phytochemicals as leading source of clinical medicines in future.

Keywords: Antioxidant; Brine shrimp lethality; Cytotoxic; Phosphomolybdate assay; Reducing power antioxidant assay; Total flavonoid contents; Total phenolic contents.