Interconversion between [2Fe-2S] and [4Fe-4S] cluster glutathione complexes

Michele Invernici; Giulia Selvolini; José Malanho Silva; Giovanna Marrazza; Simone Ciofi-Baffoni; Mario Piccioli

doi:10.1039/d1cc03566e

Interconversion between [2Fe-2S] and [4Fe-4S] cluster glutathione complexes

Chem Commun (Camb). 2022 Mar 10;58(21):3533-3536. doi: 10.1039/d1cc03566e.

Authors

Michele Invernici^{1

2}, Giulia Selvolini³, José Malanho Silva^{1

3}, Giovanna Marrazza³, Simone Ciofi-Baffoni^{1

3}, Mario Piccioli^{1

2

3}

Affiliations

¹ Magnetic Resonance Center (CERM), University of Florence, Via L. Sacconi 6, Sesto Fiorentino 50019, Italy. piccioli@cerm.unifi.it.
² Consorzio Interuniversitario Risonanze Magnetiche di Metalloproteine (CIRMMP), Via L. Sacconi 6, Sesto Fiorentino 50019, Italy.
³ Department of Chemistry "Ugo Schiff", University of Florence, Via della Lastruccia 3, Sesto Fiorentino 50019, Italy.

PMID: 35195626
DOI: 10.1039/d1cc03566e

Abstract

We present here how different iron-sulfide-glutathione ratios, applied in in vitro conditions comparable to those present in the mitochondrial matrix, affect the speciation of iron-sulfur cluster glutathione complexes. An excess of sulfide with respect to iron ions promotes the formation of a tetranuclear [Fe^II₂Fe^III₂S₄(GS)₄]^2- complex, while an excess of iron ions favors the formation of a dinuclear [Fe^IIFe^IIIS₂(GS)₄]^3- complex. These two complexes establish an interconversion equilibrium. The latter might play a role in the composition of the mitochondrial labile iron pool potentially contributing to the regulation of cellular iron homeostasis.