Degradation of chloramphenicol by Ti/PbO2-La anodes and alteration in bacterial community and antibiotics resistance genes

Hao Dong; Yanli Fu; Pengqi Wang; Wenqiang Jiang; Guangfei Gao; Xuan Zhang

doi:10.1016/j.envpol.2022.119031

Degradation of chloramphenicol by Ti/PbO₂-La anodes and alteration in bacterial community and antibiotics resistance genes

Environ Pollut. 2022 May 15:301:119031. doi: 10.1016/j.envpol.2022.119031. Epub 2022 Feb 19.

Authors

Hao Dong¹, Yanli Fu², Pengqi Wang³, Wenqiang Jiang⁴, Guangfei Gao⁵, Xuan Zhang⁶

Affiliations

¹ College of Environmental Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353, China. Electronic address: 1043119584@stu.qlu.edu.cn.
² Advanced Materials Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250014, China. Electronic address: 1043119493@stu.qlu.edu.cn.
³ College of Environmental Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353, China. Electronic address: 0431201083@stu.qlu.edu.cn.
⁴ College of Environmental Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353, China. Electronic address: jwq@qlu.edu.cn.
⁵ College of Environmental Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353, China. Electronic address: 10431201069@stu.qlu.edu.cn.
⁶ College of Environmental Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353, China. Electronic address: zx@qlu.edu.cn.

PMID: 35192886
DOI: 10.1016/j.envpol.2022.119031

Abstract

Antibiotics accumulation in the environment has given rise to multi-drug resistant 'superbugs' and antibiotics resistence genes (ARGs). Chloramphenicol (CAP), a kind of widely used antibiotics, was chosen as the model compound to investigate its degradation during electrochemical treatment process. The prepared Ti/PbO₂-La electrodes had a denser surface and a more complete PbO₂ crystal structure than Ti/PbO₂ electrode. The doping of La increased the onset potential and the overpotential, increased the current value of the oxidation peak and the reduction peak, reduced the impedance, and increased the lifetime. The reactions CAP degradation and TOC removal on Ti/PbO₂-La electrode was both primary kinetic reactions. CAP degradation rate increased with current density, and TOC obtained the highest removal at current density of 25 mA cm^-2. The electrolyte concentration had a small effect in the range of 0.050-0.150 mol L^-1. The effects under acidic and neutral conditions were better than under alkaline conditions. CAP was mainly directly oxidized at the electrode surface and indirect oxidation also took place via generated ·OH and SO₄^·-. 15 intermediates and 2 degradation pathways have been postulated. The entry of CAP and CAP intermediates into the environment caused the alteration in bacterial community and ARGs, while complete degradation products had little effect on them. Redundancy analysis showed that intI1 was the dominant factor affecting ARGs, and Actinobacteria and Patescibacteria were the main factors affecting the abundances of ARGs in the microbial community.

Keywords: Antibiotic resistance genes; Antibiotic resistant bacteria; CAP; Degradation mechanism; Ti/PbO(2)–La electrode.

MeSH terms

Anti-Bacterial Agents / pharmacology
Chloramphenicol* / analysis
Chloramphenicol* / pharmacology
Electrodes
Oxidation-Reduction
Oxides / chemistry
Titanium / chemistry
Water Pollutants, Chemical* / analysis

Substances

Anti-Bacterial Agents
Oxides
Water Pollutants, Chemical
Chloramphenicol
Titanium