Occurrence and distribution of antibiotic resistant bacteria and genes in the Fuhe urban river and its driving mechanism

Abstract

Antibiotic resistance genes (ARGs) in urban rivers can affect human health via the food chain and human pathogenic bacteria diffusion. Sediment can be a sink for ARGs, causing second sources of ARG contamination through diffusion. Therefore, we evaluated the effects of total petroleum hydrocarbons (TPHs) and phytoplankton on the distribution of the ARGs in the sediment and water of Fuhe river in Baoding city, China. The ARGs and human pathogenic bacteria in urban river were analyzed, and the phytoplankton and bacterial abundance, TPH, and physicochemical parameters ranked using the partial least squares path modelling (PLS-PM) and aggregated boosted tree (ABT) analysis. The main ARGs in Fuhe river sediment were sulfonamide and tetracycline resistance genes, with sul2 exhibiting the highest level. The main human pathogenic bacteria in the pathogens pool were Clostridium, Bacillus and Burkholderiaceae, with Clostridium demonstrating a positive correlation with SulAfolP01. The PLS-PM analysis confirmed that, among the multiple drivers, water physicochemical factors, TPH, phytoplankton, and heavy metals positively and directly affected the ARG profiles in sediment while sediment heavy metals and bacterial communities did the similar effect. These factors (nutrient factors, heavy metals, and TPH) in water and sediment posed the opposite total effect on ARGs in the sediment, suggesting medium factors should have a conclusive effect on the distribution of ARGs in the sediment. The ABT analysis showed that dissolved oxygen (DO), total nitrogen (TN) and Chlorophyta were the most important factors affecting the ARGs distribution in the water, while TN affected the distribution of the genes in the sediment.

Keywords: Antibiotic resistance genes; Pathogenic bacteria; Phytoplankton; Urban river.