Extraction of Functional Components from Freeze-Dried Angelica furcijuga Leaves Using Supercritical Carbon Dioxide

Yuriko Hoshino; Wahyudiono; Siti Machmudah; Shoji Hirayama; Hideki Kanda; Munehiro Hoshino; Motonobu Goto

doi:10.1021/acsomega.1c06105

Extraction of Functional Components from Freeze-Dried Angelica furcijuga Leaves Using Supercritical Carbon Dioxide

ACS Omega. 2022 Feb 3;7(6):5104-5111. doi: 10.1021/acsomega.1c06105. eCollection 2022 Feb 15.

Authors

Yuriko Hoshino^{1

2}, Wahyudiono¹, Siti Machmudah³, Shoji Hirayama², Hideki Kanda¹, Munehiro Hoshino⁴, Motonobu Goto¹

Affiliations

¹ Department of Materials Process Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
² M&A Food Technology and Biology of Technical Center (M.A.F.T.), Kawasaki-machi, Tagawa-gun, Fukuoka 827-0004, Japan.
³ Department of Chemical Engineering, Sepuluh Nopember Institute of Technology, Kampus ITS Sukolilo, Surabaya 60111, Indonesia.
⁴ Maruboshi Vinegar Co., Ltd., Kawasaki-machi, Tagawa-gun, Fukuoka 827-0004, Japan.

Abstract

Angelica furcijuga (A. furcijuga), as a material for traditional Chinese medicine, has been widely used in Asian countries, such as China, Korea, and Japan, for several centuries owing to its therapeutic effects. In this study, A. furcijuga leaves were used as starting materials to extract functional substances using supercritical carbon dioxide (SC-CO₂) at pressure and temperature ranges of 20-40 MPa and 40-80 °C, respectively. The extraction process was performed in a semibatch-type system with extraction times of 15-120 min. The high-performance liquid chromatography analysis indicated that kaempferol, ferulic acid, ligustilide, and butylidenephthalide as selected functional substances were successfully extracted under these operating conditions. An operating pressure of 30 MPa with an extraction time of 60 min seems to be an appropriate pressure to extract functional components from A. furcijuga leaves. The Hansen solubility parameter values and statistical analysis showed that SC-CO₂ with 10% ethanol addition is a feasible tool to isolate these selected functional substances from the A. furcijuga matrix.