Effects of enzymatic hydrolysis and alkalization pretreatment on biohydrogen production by chlorella photosynthesis

Abstract

The effects of alkalization pretreatment and enzymolysis on biohydrogen production with Chlorella vulgaris microalgae biomass by photosynthesis were studied, the alkalization pretreatment enzymolysis was to alkalize biomass raw materials before enzymolysis, the biohydrogen production kinetics equation of microalgae biomass was put forward by comparing the biohydrogen process of enzymatic hydrolysis with that of alkaline pretreatment enzymatic hydrolysis. The experimental results show: the optimum initial substrate concentration for biohydrogen production by enzymatic hydrolysis and alkaline pretreatment was 24 g/L, the maximum biohydrogen was 132.1 mL and 294.5 mL, the maximum specific biohydrogen production was 22.0 mL/g and 49.1 mL/g, and the maximum biohydrogen content was 43.9% and 56.8%. The effect of biohydrogen production by enzymatic hydrolysis after alkaline pretreatment of microalgae biomass is obviously better than that by direct enzymatic hydrolysis, which provides scientific reference and development of high efficiency and low cost biohydrogen production technology by photosynthesis of microalgae biomass.

Keywords: Alkalization pretreatment; Biohydrogen production by photosynthetic fermentation; Enzymatic hydrolysis; Microalgae; Substrate concentration.