microRNA-122 regulates NF-κB signaling pathway by targeting IκBα in miiuy croaker, Miichthys miiuy

Liyuan Yang; Weiwei Zheng; Shiying Xin; Xing Lv; Yuena Sun; Tianjun Xu

doi:10.1016/j.fsi.2022.02.025

microRNA-122 regulates NF-κB signaling pathway by targeting IκBα in miiuy croaker, Miichthys miiuy

Fish Shellfish Immunol. 2022 Mar:122:345-351. doi: 10.1016/j.fsi.2022.02.025. Epub 2022 Feb 17.

Authors

Liyuan Yang¹, Weiwei Zheng¹, Shiying Xin¹, Xing Lv¹, Yuena Sun², Tianjun Xu³

Affiliations

¹ Laboratory of Fish Molecular Immunology, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China.
² Laboratory of Fish Molecular Immunology, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources (Shanghai Ocean University), Ministry of Education, China; National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, 201306, China. Electronic address: yuenasun@163.com.
³ Laboratory of Fish Molecular Immunology, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China; Laboratory of Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources (Shanghai Ocean University), Ministry of Education, China; National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, 201306, China. Electronic address: tianjunxu@163.com.

PMID: 35182723
DOI: 10.1016/j.fsi.2022.02.025

Abstract

The inhibitory protein IκBα plays a key role in the inflammatory process and immune response by regulating the activity of the transcription factor NF-κB. microRNA (miR) is a small non-coding RNA that can regulate many biochemical processes, such as cell growth, proliferation, and immune response. In this study, it was first predicted that IκBα is the target of miR-122 through bioinformatics, and it was confirmed by dual fluorescence experiments. Then we found that miR-122 can inhibit the expression of IκBα at the mRNA and protein levels, thereby promoting the p65-activated NF-κB pathway. It is speculated that miR-122 plays an important role in the innate immunity of teleost fish. This study will help to further understand miRNAs regulatory mechanism in teleost fish.

Keywords: Innate immune; IκBα; NF-κB signaling; Negatively regulate; microRNA.

MeSH terms

Animals
Fish Proteins
Immunity, Innate / genetics
MicroRNAs* / metabolism
NF-KappaB Inhibitor alpha / genetics
NF-KappaB Inhibitor alpha / metabolism
NF-kappa B / metabolism
Perciformes*
Signal Transduction

Substances

Fish Proteins
MicroRNAs
NF-kappa B
NF-KappaB Inhibitor alpha