Iron from nanostructured ferric phosphate: absorption and biodistribution in mice and bioavailability in iron deficient anemic women

Abstract

Food fortification with iron nanoparticles (NPs) could help prevent iron deficiency anemia, but the absorption pathway and biodistribution of iron-NPs and their bioavailability in humans is unclear. Dietary non-heme iron is physiologically absorbed via the divalent metal transporter-1 (DMT1) pathway. Using radio- iron isotope labelling in mice with a partial knockdown of intestine-specific DMT1, we assessed oral absorption and tissue biodistribution of nanostructured ferric phosphate (FePO₄-NP; specific surface area [SSA] 98 m²g^-1) compared to to ferrous sulfate (FeSO₄), the reference compound. We show that absorption of iron from FePO₄-NP appears to be largely DMT1 dependent and that its biodistribution after absorption is similar to that from FeSO₄, without abnormal deposition of iron in the reticuloendothelial system. Furthermore, we demonstrate high bioavailability from iron NPs in iron deficient anemic women in a randomized, cross-over study using stable-isotope labelling: absorption and subsequent erythrocyte iron utilization from two ⁵⁷Fe-labeled FePO₄-NP with SSAs of 98 m²g^-1 and 188 m²g^-1 was 2.8-fold and 5.4-fold higher than from bulk FePO₄ with an SSA of 25 m²g^-1 (P < 0.001) when added to a rice and vegetable meal consumed by iron deficient anemic women. The FePO₄-NP 188 m²g^-1 achieved 72% relative bioavailability compared to FeSO₄. These data suggest FePO₄-NPs may be useful for nutritional applications.