Kefir modulates gut microbiota and reduces DMH-associated colorectal cancer via regulation of intestinal inflammation in adulthood offsprings programmed by neonatal overfeeding

Poliana Guiomar de Almeida Brasiel; Sheila Cristina Potente Dutra Luquetti; Julliane Dutra Medeiros; José Otavio do Amaral Corrêa; Alessandra Barbosa Ferreira Machado; Ana Paula Boroni Moreira; Vinícius Novaes Rocha; Claudio Teodoro de Souza; Maria do Carmo Gouveia Peluzio

doi:10.1016/j.foodres.2021.110708

Kefir modulates gut microbiota and reduces DMH-associated colorectal cancer via regulation of intestinal inflammation in adulthood offsprings programmed by neonatal overfeeding

Food Res Int. 2022 Feb:152:110708. doi: 10.1016/j.foodres.2021.110708. Epub 2021 Sep 8.

Authors

Poliana Guiomar de Almeida Brasiel¹, Sheila Cristina Potente Dutra Luquetti², Julliane Dutra Medeiros³, José Otavio do Amaral Corrêa⁴, Alessandra Barbosa Ferreira Machado⁵, Ana Paula Boroni Moreira⁶, Vinícius Novaes Rocha⁷, Claudio Teodoro de Souza⁸, Maria do Carmo Gouveia Peluzio¹

Affiliations

¹ Department of Nutrition and Health, Federal University of Viçosa, Viçosa, Minas Gerais, Brazil.
² Department of Nutrition, Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil. Electronic address: sheila.dutra@ufjf.edu.br.
³ Faculty of Biological and Agricultural Sciences, Mato Grosso State University, Alta Floresta, Brazil.
⁴ Department of Pharmaceutical Sciences, Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil.
⁵ Department of Parasitology, Microbiology and Immunology, Federal University of Juiz de Fora, Brazil.
⁶ Department of Nutrition, Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil.
⁷ Department of Veterinary Medicine, Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil.
⁸ Department of Clinical Medicine, Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil.

PMID: 35181109
DOI: 10.1016/j.foodres.2021.110708

Abstract

Obesity is associated with chronic inflammation, intestinal dysbiosis, and colorectal cancer risk. The anti-cancer effects of kefir are highlighted. Here, lactating Wistar rats were divided into: Normal litter (NL); Kefir normal litter (KNL); Small litter (SL); Kefir small litter (KSL). The NL and SL groups received 1 mL of water/day; KNL and KSL received kefir milk daily (10⁸ CFU/mL) during lactation. After weaning, the pups continued to receive the same treatments until 60 days. At 67 days old, colorectal carcinogenesis was induced through intraperitoneal injection of 1,2-dimethylhydrazine. At 240 days, visceral adipose tissue was higher in SL compared to NL, KNL, and KSL. Kefir intake was found to suppress the number of tumors in both KNL and KSL groups (-100% and -71.43%; p < 0.01, respectively). IL-1β, IL-6, TNF-α, and NO levels in the colon were higher in the NL and SL compared to the KNL and KSL. The gut microbiota in cecal samples of SL was enriched with Alloprevotella, Acinetobacter, and Bacteroides. In contrast, the cecal contents of KSL and KNL were higher Romboutsia. Thus, neonatal overfeeding leads to greater adiposity, inflammation and number of colon tumors in adulthood. Early-life nutrition based on kefir reverted these alterations.

Keywords: Adiposity; Colorectal neoplasms; Inflammation; Kefir; Microbiota; Probiotics.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Colorectal Neoplasms* / prevention & control
Female
Gastrointestinal Microbiome*
Inflammation
Kefir*
Lactation
Rats
Rats, Wistar