Validating RRP12 Expression and Its Prognostic Significance in HCC Based on Data Mining and Bioinformatics Methods

Chao Wei; Ben Wang; Zhong-Huo Chen; Han Xiao; Lei Tang; Jia-Fu Guan; Rong-Fa Yuan; Xin Yu; Zhi-Gang Hu; Hua-Jun Wu; Zhi Dai; Kai Wang

doi:10.3389/fonc.2022.812009

Validating RRP12 Expression and Its Prognostic Significance in HCC Based on Data Mining and Bioinformatics Methods

Front Oncol. 2022 Feb 1:12:812009. doi: 10.3389/fonc.2022.812009. eCollection 2022.

Authors

Chao Wei^{1

2

3}, Ben Wang^{1

2

3}, Zhong-Huo Chen^{1

2

3}, Han Xiao⁴, Lei Tang^{1

2

3}, Jia-Fu Guan^{1

3}, Rong-Fa Yuan^{1

3

5}, Xin Yu^{1

3

5}, Zhi-Gang Hu^{1

3

5}, Hua-Jun Wu^{1

3

5}, Zhi Dai⁶, Kai Wang^{1

3

5}

Affiliations

¹ Hepato-Biliary-Pancreatic Surgery Division, Department of General Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, China.
² Second Affiliated Hospital of Nanchang University, Jiangxi Province Key Laboratory of Molecular Medicine, Nanchang, China.
³ Second Affiliated Hospital of Nanchang University, Jiangxi Province Engineering Research Center of Hepatobiliary Disease, Nanchang, China.
⁴ Department of Hepato-Biliary-Pancreatic Surgery, Jiujiang First People's Hospital, Jiujiang, China.
⁵ Second Affiliated Hospital of Nanchang University, Jiangxi Provincial Clinical Research Center for General Surgery Disease, Nanchang, China.
⁶ Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai, China.

Abstract

RRP12 (ribosomal RNA processing 12 homolog) is a nucleolar protein involved in the maturation and transport of eukaryotic ribosomal subunits and is a type of RNA binding protein. In recent years, considerable research has indicated that RRP12 is associated with the occurrence and development of multiple cancers. However, there is no research on RRP12 in hepatocellular carcinoma. Herein, we aimed to explore the role and significance of RRP12 in hepatocellular carcinoma.We used the TIMER and GEPIA databases to perform pan-cancer analyses of RRP12. The impact of RRP12 on the prognosis was analyzed through the GEPIA database. The relationship between RRP12 and immune cell infiltration was investigated by TIMER and GEPIA databases. Moreover, the expression of RRP12 in various liver cancer cells was evaluated by Western Blot to determine the cell line for the next experiment. Scratch test, Transwell test, and Edu tests were applied to validate the effects of RRP12 on the function of liver cancer cells. And the data were statistically analyzed.Pan-cancer analysis found that RPP12 was significantly upregulated in many cancers. Moreover, the prognostic analysis revealed that the difference in the expression of RRP12 has statistical significance for the overall survival rate and disease-free survival rate of liver cancer patients. In order to analyze the correlation between the expression level of RRP12 and clinical parameters, it was found that there was a significant negative correlation with tumor stage, tumor grade and tumor size. Univariate and multivariate analysis showed that RRP12 could be used as an independent prognostic factor for patients with hepatocellular carcinoma. Cellular experiments have proved that knocking down RRP12 can inhibit the proliferation, invasion, and metastasis of liver cancer cells.Therefore, RRP12 significantly affects the occurrence and development of HCC. Hence, RRP12 can become a potential target and prognostic biomarker for the treatment of hepatocellular carcinoma.

Keywords: RRP12; hepatocellular carcinoma; molecule biomarkers; prognosis; tumor immune infiltration.