An efficient multi-enzyme cascade platform based on mesoporous metal-organic frameworks for the detection of organophosphorus and glucose

Xiyue Cao; Yanmei Guo; Mu Zhao; Jie Li; Chonglin Wang; Jianfei Xia; Tingting Zou; Zonghua Wang

doi:10.1016/j.foodchem.2022.132282

An efficient multi-enzyme cascade platform based on mesoporous metal-organic frameworks for the detection of organophosphorus and glucose

Food Chem. 2022 Jul 1:381:132282. doi: 10.1016/j.foodchem.2022.132282. Epub 2022 Jan 31.

Authors

Xiyue Cao¹, Yanmei Guo¹, Mu Zhao², Jie Li², Chonglin Wang³, Jianfei Xia⁴, Tingting Zou⁵, Zonghua Wang⁶

Affiliations

¹ College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Instrumental Analysis Center of Qingdao University, Qingdao University, Qingdao 266071, PR China.
² Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology & Business University (BTBU), Beijing 100048, PR China.
³ Centre Testing International (Qingdao) Co., Ltd, Qingdao 266071, PR China.
⁴ College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Instrumental Analysis Center of Qingdao University, Qingdao University, Qingdao 266071, PR China. Electronic address: xiajianfei@126.com.
⁵ Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology & Business University (BTBU), Beijing 100048, PR China. Electronic address: zoutingting@th.btbu.edu.cn.
⁶ College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Instrumental Analysis Center of Qingdao University, Qingdao University, Qingdao 266071, PR China. Electronic address: wangzonghua@qdu.edu.cn.

PMID: 35176684
DOI: 10.1016/j.foodchem.2022.132282

Abstract

An efficient colorimetric detection platform based on multi-enzyme cascade has been developed for detection of organophosphorus. Firstly, the dual-enzyme platform was prepared and applied for sensitive glucose detection (detection limit 0.32 μM). And then three enzymes, including acetylcholinesterase, horseradish peroxidase and choline oxidase were encapsulated in cruciate flower-like zeolitic imidazolate framework-8 (CF-ZIF-8) through one-step co-precipitation to construct detection platform with acetylcholine chloride as substrate. The acephate inhibited the activity of acetylcholinesterase, obstructed the cascade reaction and reduced the production of H₂O₂, resulting in the changes of color intensity for the colorimetric detection. With suitable size and porous structure, CF-ZIF-8 provided a good microenvironment for guaranteeing the activity and spatial proximity of enzymes. The multi-enzyme platform displayed great performances with the detection limit of 0.23 nM for acephate. It was applied to the detection of acephate in Chinese cabbage and romaine, verifying the practicability of this platform.

Keywords: Acephate; Colorimetric sensor; Cruciate flower-like ZIF-8; Multi-enzyme cascade; Organophosphorus.

MeSH terms

Acetylcholinesterase
Biosensing Techniques* / methods
Colorimetry
Glucose
Hydrogen Peroxide / chemistry
Metal-Organic Frameworks* / chemistry

Substances

Metal-Organic Frameworks
Hydrogen Peroxide
Acetylcholinesterase
Glucose