Transcription by RNA polymerase II and the CTD-chromatin crosstalk

Neha Singh; Mohd Asalam; Mohd Owais Ansari; Nadezhda S Gerasimova; Vasily M Studitsky; Md Sohail Akhtar

doi:10.1016/j.bbrc.2022.02.039

Transcription by RNA polymerase II and the CTD-chromatin crosstalk

Biochem Biophys Res Commun. 2022 Apr 9:599:81-86. doi: 10.1016/j.bbrc.2022.02.039. Epub 2022 Feb 11.

Authors

Neha Singh¹, Mohd Asalam¹, Mohd Owais Ansari¹, Nadezhda S Gerasimova², Vasily M Studitsky², Md Sohail Akhtar³

Affiliations

¹ Biochemistry and Structural Biology Division, CSIR-Central Drug Research Institute, Lucknow, India.
² Department of Bioengineering, School of Biology, Lomonosov Moscow State University, Moscow, Russia.
³ Biochemistry and Structural Biology Division, CSIR-Central Drug Research Institute, Lucknow, India. Electronic address: sohail@cdri.res.in.

PMID: 35176629
DOI: 10.1016/j.bbrc.2022.02.039

Abstract

The epigenetic phenomenon is known to derive the phenotypic variation of an organism through an interconnected cellular network of histone modifications, DNA methylation and RNA regulatory network. Transcription for protein coding genes is a highly regulated process and carried out by a large multi-complex RNA Polymerase II. The carboxy terminal domain (CTD) of the largest subunit of RNA Polymerase II consists of a conserved and highly repetitive heptad sequence Tyr₁-Ser₂-Pro₃-Thr₄-Ser₅-Pro₆-Ser₇. The epigenetically modified CTD is thought to selectively bind different protein complexes that participate in mRNA biogenesis and export. The CTD and chromatin appears to have a spatial relationship during the transcription cycle, where the epigenetic modifications of CTD not only influence the state of histone modification but also mediates CTD-chromatin crosstalk. In this mini review, we have surveyed and discussed current developments of RNA Polymerase II CTD and its new emerging crosstalk with chromatin, during the stage specific progression of RNA Polymerase II in transcription cycle. This review is mainly focussed on the insights in budding yeast.

Keywords: CTD; Chromatin; Kinases; RNA polymerase II; Transcription.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Chromatin / genetics
Chromatin / metabolism*
Phosphorylation
Protein Domains
RNA Polymerase II / chemistry
RNA Polymerase II / genetics
RNA Polymerase II / metabolism*
Saccharomycetales / genetics
Saccharomycetales / metabolism
Transcription, Genetic*

Substances

Chromatin
RNA Polymerase II