Emerging fluorescence tools for the study of proteostasis in cells

Tze Cin Owyong; Yuning Hong

doi:10.1016/j.cbpa.2022.102116

Emerging fluorescence tools for the study of proteostasis in cells

Curr Opin Chem Biol. 2022 Apr:67:102116. doi: 10.1016/j.cbpa.2022.102116. Epub 2022 Feb 14.

Authors

Tze Cin Owyong¹, Yuning Hong²

Affiliations

¹ Department of Biochemistry and Chemistry, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, 3086, Australia; ARC Centre of Excellence in Exciton Science, School of Chemistry, Bio21 Institute, The University of Melbourne, Victoria, 3010, Australia.
² Department of Biochemistry and Chemistry, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, 3086, Australia. Electronic address: y.hong@latrobe.edu.au.

PMID: 35176555
DOI: 10.1016/j.cbpa.2022.102116

Abstract

Understanding how cells maintain the functional proteome and respond to stress conditions is critical for deciphering molecular pathogenesis and developing treatments for conditions such as neurodegenerative diseases. Efforts towards finer quantification of cellular proteostasis machinery efficiency, phase transitions and local environment changes remain a priority. Herein, we describe recent developments in fluorescence-based strategy and methodology, building on the experimental toolkit, for the study of proteostasis (protein homeostasis) in cells. We hope this review can assist in bridging gaps between a multitude of research disciplines and promote interdisciplinary collaboration to address the crucial topic of proteostasis.

Keywords: Fluorescence imaging; Fluorescent probes; Protein aggregation; Protein misfolding; Proteostasis.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Fluorescence
Humans
Protein Folding
Proteome / metabolism
Proteostasis Deficiencies* / pathology
Proteostasis*

Substances

Proteome