Assessing the availability of two bamboo species for fermentable sugars by alkaline hydrogen peroxide pretreatment

Yunni Zhan; Jinyuan Cheng; Xuze Liu; Chen Huang; Jia Wang; Shanming Han; Guigan Fang; Xianzhi Meng; Arthur J Ragauskas

doi:10.1016/j.biortech.2022.126854

Assessing the availability of two bamboo species for fermentable sugars by alkaline hydrogen peroxide pretreatment

Bioresour Technol. 2022 Apr:349:126854. doi: 10.1016/j.biortech.2022.126854. Epub 2022 Feb 14.

Authors

Yunni Zhan¹, Jinyuan Cheng¹, Xuze Liu¹, Chen Huang², Jia Wang³, Shanming Han¹, Guigan Fang⁴, Xianzhi Meng⁵, Arthur J Ragauskas⁶

Affiliations

¹ Institute of Chemical Industry of Forest Products, Chinese Academy of Forestry, Jiangsu Province Key Laboratory of Biomass Energy and Materials, Nanjing 210042, China.
² Institute of Chemical Industry of Forest Products, Chinese Academy of Forestry, Jiangsu Province Key Laboratory of Biomass Energy and Materials, Nanjing 210042, China; Co-Innovation Center for Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037, China. Electronic address: huangchen3127@njfu.edu.cn.
³ Co-Innovation Center for Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037, China.
⁴ Institute of Chemical Industry of Forest Products, Chinese Academy of Forestry, Jiangsu Province Key Laboratory of Biomass Energy and Materials, Nanjing 210042, China; Co-Innovation Center for Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037, China.
⁵ Department of Chemical and Biomolecular Engineering, University of Tennessee Knoxville, Knoxville, TN 37996, USA.
⁶ Department of Chemical and Biomolecular Engineering, University of Tennessee Knoxville, Knoxville, TN 37996, USA; Department of Forestry, Wildlife, and Fisheries, Center for Renewable Carbon, The University of Tennessee Institute of Agriculture, Knoxville, TN 37996, USA; Joint Institute for Biological Science, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA.

PMID: 35176465
DOI: 10.1016/j.biortech.2022.126854

Abstract

This study comprehensively investigated two bamboo species (i.e. Neosinocalamus affinis and Phyllostachys edulis) in terms of their cell wall ultrastructure, chemical compositions, enzymatic saccharification, and lignin structure before and after alkaline hydrogen peroxide pretreatment (AHP). During AHP, Neosinocalamus affinis (NAB) had higher delignification than Phyllostachys edulis (PEB), and thus showed better enzymatic digestibility (93.05% vs 53.57% for glucan). The fundamental chemical behavior of the bamboo lignins was analyzed by fluorescence microscope (FM), confocal Raman microscope (CRM), molecular weight analysis, and 2D HSQC-NMR. Results indicated that the PEB has thicker cell wall and more concentrated lignin in its compound middle lamella and cell corner middle lamella than NAB. Moreover, PEB lignin contains more G units (S/G of 0.95), in evident contrast to that of NAB lignin (S/G of 1.30), which favor the formation of C-C linkages, thus impeding its degradation during the AHP.

Keywords: Alkaline hydrogen peroxide pretreatment; Enzymatic saccharification; Lignin; Neosinocalamus affinis; Phyllostachys edulis.

MeSH terms

Bambusa* / chemistry
Hydrogen Peroxide*
Hydrolysis
Lignin / chemistry
Sugars

Substances

Sugars
Lignin
Hydrogen Peroxide