Lineage Tracing Methods to Study Mammary Epithelial Hierarchies In Vivo

Verónica Rodilla; Silvia Fre

doi:10.1007/978-1-0716-2193-6_7

Lineage Tracing Methods to Study Mammary Epithelial Hierarchies In Vivo

Methods Mol Biol. 2022:2471:141-157. doi: 10.1007/978-1-0716-2193-6_7.

Authors

Verónica Rodilla¹, Silvia Fre²

Affiliations

¹ Cancer Heterogeneity and Hierarchies Group, Josep Carreras Leukaemia Research Institute (IJC), Badalona, Spain.
² Department of Genetics and Developmental Biology, Institut Curie, PSL Research University, INSERM U934, CNRS UMR3215, Paris, France. silvia.fre@curie.fr.

PMID: 35175595
DOI: 10.1007/978-1-0716-2193-6_7

Abstract

Lineage tracing is now considered the gold standard approach to study cellular hierarchies and cell fate in vivo (McKenna and Gagnon, Development 146:dev169730, 2019; Kretzschmar and Watt, Cell 148:33-45, 2012). This type of clonal analysis consists of genetically labeling defined cells and following their destiny and progeny in vivo and in situ.Here we will describe different existing in vivo systems to clonally trace targeted cells and will discuss their respective advantages and inconveniences; we will then provide stepwise instructions for setting up and evaluate lineage tracing experiments, listing the most common downstream analyses and read-out assays.

Keywords: Cellular hierarchies; Inducible Cre/Lox system; Ineage tracing; Stem cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Breast* / cytology
Cell Lineage*