Xylaria feejeensis, SRNE2BP a Fungal Endophyte with Biocontrol Properties to Control Early Blight and Fusarium Wilt Disease in Tomato and Plant Growth Promotion Activity

Abstract

Over the past decade endophytic fungi have been known as a source of secondary metabolites with the ability to act as a biocontrol agents. Xylaria feejeensis, SRNE2BP a fungal endophyte isolated from a mangrove tree exhibited antagonistic activity against two fungal pathogens of tomato. Crude extract of X. feejeensis SRNE2BP significantly inhibited Fusarium oxysporum MFLUCC 19-0157 growth as shown approximately 60-75% in in vitro and in situ assays. Both assays showed that the endophyte also inhibited mycelium formation of Alternaria solani MFLUCC 19-0093 by 56% and 87%, respectively. The half maximal inhibitory concentration of X. feejeensis SRNE2BP crude extract against A. solani and F. oxysporum was approximately 7 mg/l. Crude extract and mycelium of X. feejeensis SRNE2BP showed potential in controlling early blight and fusarium wilt disease in tomato, respectively. Seedlings from seeds coated with crude extract of X. feejeensis SRNE2BP had lower disease severity (31.71%) of early blight disease compared to un-treated seeds (57.13%). Soil treated with 10% endophytic mycelium not only reduced fusarium wilt in tomato plant (55.55% severity compared with 91.66% in un-treated soil) but also promoted seed emergence and growth of tomato. Structure analysis revealed that 12 secondary metabolites, especially mellein derivatives, are major components of the crude extract of X. feejeensis SRNE2BP. These compounds could be responsible for antifungal activities; however, further study is required. Our findings strongly suggest that colonization with this fungal endophyte can be beneficial to the host plant especially with regards to plant growth promotion and broad antagonistic activity.