Lateral flow immunoassay for small-molecules detection in phytoproducts: a review

J Nat Med. 2022 Jun;76(3):521-545. doi: 10.1007/s11418-022-01605-6. Epub 2022 Feb 16.

Abstract

Phytoproducts are involved in various fields of industry. Small-molecule (Mw < 900 Da) organic compounds can be used to indicate the quality of plant samples in the perspective of efficacy by measuring the necessary secondary metabolites and in the perspective of safety by measuring the adulterant level of toxic compounds. The development of reliable detection methods for these compounds in such a complicated matrix is challenging. The lateral flow immunoassay (LFA) is one of the immunoassays well-known for its simplicity, portability, and rapidity. In this review, the general principle, components, format, and application of the LFA for phytoproducts are discussed.

Keywords: Hapten; Immunoassay; Immunochromatographic strip test; Lateral flow immunoassay; Plant secondary metabolites; Small molecules.

Publication types

  • Review

MeSH terms

  • Immunoassay* / methods
  • Phytochemicals* / isolation & purification

Substances

  • Phytochemicals