Enzymatic glycoengineering-based spin labelling of cell surface sialoglycans to enable their analysis by electron paramagnetic resonance (EPR) spectroscopy

Mohit Jaiswal; Trang T Tran; Jiatong Guo; Mingwei Zhou; Josefina Garcia Diaz; Gail E Fanucci; Zhongwu Guo

doi:10.1039/d1an02226a

Enzymatic glycoengineering-based spin labelling of cell surface sialoglycans to enable their analysis by electron paramagnetic resonance (EPR) spectroscopy

Analyst. 2022 Feb 28;147(5):784-788. doi: 10.1039/d1an02226a.

Authors

Mohit Jaiswal¹, Trang T Tran¹, Jiatong Guo¹, Mingwei Zhou¹, Josefina Garcia Diaz¹, Gail E Fanucci¹, Zhongwu Guo¹

Affiliation

¹ Department of Chemistry, University of Florida, 214 Leigh Hall, Gainesville, FL 32611, USA. zguo@chem.ufl.edu.

Abstract

A novel method for spin labelling of sialoglycans on the cell surface is described. C9-Azido sialic acid was linked to glycans on live cells via CSTII-catalysed α2,3-sialylation utilizing azido-sialic acid nucleotide as a sialyl donor, which was followed by attachment of a spin label to the azide via click reaction. It enables the study of cell surface sialoglycans by EPR spectroscopy.

MeSH terms

Azides*
Cell Membrane / metabolism
Electron Spin Resonance Spectroscopy / methods
Polysaccharides* / chemistry
Spin Labels

Substances

Azides
Polysaccharides
Spin Labels

Grants and funding

R35 GM131686/GM/NIGMS NIH HHS/United States