Activation of UCP1-Independent Ca2+ Cycling Thermogenesis by Wireless Optogenetics

Kenji Ikeda; Kazuki Tajima; Yuji Tanabe; Ada S Y Poon; Shingo Kajimura

doi:10.1007/978-1-0716-2087-8_9

Activation of UCP1-Independent Ca²⁺ Cycling Thermogenesis by Wireless Optogenetics

Methods Mol Biol. 2022:2448:131-139. doi: 10.1007/978-1-0716-2087-8_9.

Authors

Kenji Ikeda¹, Kazuki Tajima², Yuji Tanabe³, Ada S Y Poon^{4

5}, Shingo Kajimura⁶

Affiliations

¹ Department of Molecular Endocrinology and Metabolism, Tokyo Medical and Dental University, Tokyo, Japan.
² Department of Endocrinology and Metabolism, Yokohama Medical Center, Yokohama, Japan.
³ Department of Electrical Engineering, Stanford University, Stanford, CA, USA.
⁴ Department of Electrical Engineering, Stanford University, Stanford, CA, USA. adapoon@stanford.edu.
⁵ Chan Zuckerberg Biohub, San Francisco, CA, USA. adapoon@stanford.edu.
⁶ Division of Endocrinology, Diabetes and Metabolism, Beth Israel Deaconess Medical Center, Harvard Medical School, Harvard, MA, USA. skajimur@bidmc.harvard.edu.

Abstract

The identification of non-canonical UCP1-independent thermogenic mechanisms offers new opportunities to target such pathways to improve metabolic health. Based on our recent studies on Ca²⁺ futile cycling thermogenesis in beige fat, we applied the newly developed implantable wireless optogenetic system to activate Ca²⁺ cycling in an adipocyte-specific manner without external stimuli, i.e., fat-specific cold mimetics. Here, we describe the detailed methodology and application to the prevention of obesity.

Keywords: Beige fat; Ca2+ cycling thermogenesis; Obesity; Optogenetics; SERCA2.

MeSH terms

Adipocytes / metabolism
Adipose Tissue, Beige / metabolism
Energy Metabolism
Humans
Obesity / metabolism
Optogenetics*
Thermogenesis*
Uncoupling Protein 1 / genetics
Uncoupling Protein 1 / metabolism

Substances

UCP1 protein, human
Uncoupling Protein 1

Grants and funding

R01 DK097441/DK/NIDDK NIH HHS/United States