Fluorogenic Detection of Human Serum Albumin Using Curcumin-Capped Mesoporous Silica Nanoparticles

Molecules. 2022 Feb 8;27(3):1133. doi: 10.3390/molecules27031133.

Abstract

Mesoporous silica nanoparticles loaded with rhodamine B and capped with curcumin are used for the selective and sensitive fluorogenic detection of human serum albumin (HSA). The sensing mesoporous silica nanoparticles are loaded with rhodamine B, decorated with aminopropyl moieties and capped with curcumin. The nanoparticles selectively release the rhodamine B cargo in the presence of HSA. A limit of detection for HSA of 0.1 mg/mL in PBS (pH 7.4)-acetonitrile 95:5 v/v was found, and the sensing nanoparticles were used to detect HSA in spiked synthetic urine samples.

Keywords: HSA detection; gated hybrid materials; mesoporous silica; sensors.

MeSH terms

  • Curcumin / chemistry*
  • Fluorescent Dyes / chemistry*
  • Humans
  • Nanoparticles / chemistry*
  • Rhodamines / chemistry*
  • Serum Albumin, Human / analysis
  • Serum Albumin, Human / urine*
  • Silicon Dioxide / chemistry*
  • Spectrometry, Fluorescence

Substances

  • Fluorescent Dyes
  • Rhodamines
  • Silicon Dioxide
  • Curcumin
  • rhodamine B
  • Serum Albumin, Human