The second edition of this Special Issue "Strategies and Evidence in Health Communication", published in the International Journal of Environmental Research and Public Health aims primarily to increase international literature evidence and observations in the field regarding: (i) health communication strategies and crisis communication, (ii) health education and health advocacy, and (iii) the fight against the phenomenon of Vaccine Hesitancy (VH) through training and communication activities targeting the general public and health professionals. This Special Issue builds on the premise that, despite the fact that theoretical and experimental research has contributed to an increase in knowledge and evidence about the importance of communication in healthcare, communication professionals in this field still face great challenges when trying to develop messages that effectively change the behavior of large groups of people. The need to relay fast and reliable information to the general public has therefore led public institutions to seek out new and innovative ways of transmitting health-related content. In particular, for some time now, Public Health has also been making use of the Internet and Information and Communication Technologies (ICT) to reach various population groups and achieve better health conditions for all. This practice, known as Digital Health or E-health, provides healthcare using digital tools (e.g., websites and social media networks) and easy-to-understand language. This is particularly important in the current pandemic context, where Public Health continues to face many problems and difficulties in persuading people to adhere to the guidelines issued for the containment of COVID-19, with particular reference to vaccination programs, hence the importance of acquiring and strengthening communication skills in healthcare, where correct and effective communication is immediately beneficial both to professionals and patients.