Impact of APRF+ in Combination with Autogenous Fibroblasts on Release Growth Factors, Collagen, and Proliferation and Migration of Gingival Fibroblasts: An In Vitro Study

Barbara Sterczała; Agnieszka Chwiłkowska; Urszula Szwedowicz; Magdalena Kobielarz; Bartłomiej Chwiłkowski; Marzena Dominiak

doi:10.3390/ma15030796

Impact of APRF+ in Combination with Autogenous Fibroblasts on Release Growth Factors, Collagen, and Proliferation and Migration of Gingival Fibroblasts: An In Vitro Study

Materials (Basel). 2022 Jan 21;15(3):796. doi: 10.3390/ma15030796.

Authors

Barbara Sterczała¹, Agnieszka Chwiłkowska², Urszula Szwedowicz², Magdalena Kobielarz³, Bartłomiej Chwiłkowski⁴, Marzena Dominiak¹

Affiliations

¹ Dental Surgery Department, Wroclaw Medical University, 50-425 Wroclaw, Poland.
² Department of Molecular and Cell Biology, Wroclaw Medical University, 50-556 Wroclaw, Poland.
³ Department of Mechanics, Materials and Biomedical Engineering, Wroclaw University of Science and Technology, 50-371 Wroclaw, Poland.
⁴ Department of Applied Mathematics, Faculty of Pure and Applied Mathematics, Wroclaw University of Science and Technology, 50-370 Wroclaw, Poland.

Abstract

The present study aimed to compare the action of advanced platelet-rich fibrin (A-PRF+) alone with the action of A-PRF+ combined with autologous gingival fibroblasts. The components released from A-PRF+ conditioned with autogenous fibroblasts that were quantified in the study were fibroblast growth factor (FGF), vascular endothelial growth factor (VEGF), trans-forming growth factor-beta1 and 2 (TGFβ1 and TGFβ2), and soluble collagen. A-PRF+ combined with fibroblasts demonstrated significantly higher values of released VEGF at every time point and, after 7 days, significantly higher values of released TGFβ2. A viability test after 72 h showed a significant increase in proliferation fibroblasts after exposition to the factors released from A-PRF+ combined with fibroblasts. Similarly, the degree of wound closure after 48 h was significantly higher for the factors released from A-RRF+ alone and the factors released from A-RRF+ combined with fibroblasts. These results imply that platelet-rich fibrin (PRF) enhanced with fibroblasts can be an alternative method of connective tissue transplantation.

Keywords: A-PRF+; TGFβ2; VEGF; fibroblast culture; wound healing.

Grants and funding

STM.B040.20.078; PI; 016/RID/2018/19/Wroclaw Medical University, Poland; the Ministry of Science and Higher Education in the "Regional Initiative of Excellence"