Functional Nanoparticles with Magnetic 3D Covalent Organic Framework for the Specific Recognition and Separation of Bovine Serum Albumin

Lokesh Bettada; Hweiyan Tsai; C Bor Fuh

doi:10.3390/nano12030411

Functional Nanoparticles with Magnetic 3D Covalent Organic Framework for the Specific Recognition and Separation of Bovine Serum Albumin

Nanomaterials (Basel). 2022 Jan 26;12(3):411. doi: 10.3390/nano12030411.

Authors

Lokesh Bettada¹, Hweiyan Tsai^{2

3}, C Bor Fuh¹

Affiliations

¹ Department of Applied Chemistry, National Chi Nan University, Nantou 545, Taiwan.
² Department of Medical Applied Chemistry, Chung Shan Medical University, Taichung 402, Taiwan.
³ Department of Medical Education, Chung Shan Medical University Hospital, Taichung 402, Taiwan.

Abstract

Glutathione functionalized magnetic 3D covalent organic frameworks combined with molecularly imprinted polymer (magnetic 3D COF-GSH MIPs) were developed for the selective recognition and separation of bovine serum albumin (BSA). Ultrasonication was used to prepare magnetic 3D COFs with high porosity (~1 nm) and a large surface area (373 m² g^-1). The magnetic 3D COF-GSH MIP nanoparticles had an imprinting factor of 4.79, absorption capacity of 429 mg g^-1, magnetic susceptibility of 32 emu g^-¹, and five adsorption-desorption cycles of stability. The proposed method has the advantages of a shorter equilibrium absorption time (1.5 h), higher magnetic susceptibility (32 emu g^-1), and larger imprinting factor (4.79) compared with those reported from other studies. The magnetic 3D COF-GSH MIPs used with BSA had selectivity factors of 3.68, 2.76, and 3.30 for lysozyme, ovalbumin, and cytochrome C, respectively. The successful recognition and separation of BSA in a real sample analysis verified the capability of the magnetic 3D COF-GSH MIP nanoparticles.

Keywords: bovine serum albumin; magnetic 3D covalent organic frameworks; protein imprinted polymer.

Grants and funding

MOST-108-2113-M-260-009/Ministry of Science and Technology in Taiwan