Studies of interactions between fibronectin and a specific antibody against fibronectin using SPRi and QCM

Lukasz Oldak; Zenon Lukaszewski; Ewa Gorodkiewicz

doi:10.1016/j.jpba.2022.114640

Studies of interactions between fibronectin and a specific antibody against fibronectin using SPRi and QCM

J Pharm Biomed Anal. 2022 Apr 1:212:114640. doi: 10.1016/j.jpba.2022.114640. Epub 2022 Feb 1.

Authors

Lukasz Oldak¹, Zenon Lukaszewski², Ewa Gorodkiewicz³

Affiliations

¹ Doctoral School of Exact and Natural Science, Faculty of Chemistry, Bioanalysis Laboratory, University of Bialystok, Ciolkowskiego 1 K, 15-245 Bialystok, Poland; Bioanalysis Laboratory, Faculty of Chemistry, University of Bialystok, Ciolkowskiego 1 K, 15-245 Bialystok, Poland. Electronic address: l.oldak@uwb.edu.pl.
² Faculty of Chemical Technology, Poznan University of Technology, pl. Sklodowskiej-Curie 5, 60-965 Poznan, Poland. Electronic address: zenon.lukaszewski@put.edu.pl.
³ Bioanalysis Laboratory, Faculty of Chemistry, University of Bialystok, Ciolkowskiego 1 K, 15-245 Bialystok, Poland. Electronic address: ewka@uwb.edu.pl.

PMID: 35158185
DOI: 10.1016/j.jpba.2022.114640

Abstract

Surface Plasmon Resonance Imaging (SPRi) and the Quartz Crystal Microbalance (QCM) technique were used to characterize the interactions between fibronectin and a specific monoclonal antibody against fibronectin. These techniques were used to investigate the formation of successive layers of the biosensor and how they affect the biosensor's response. The analytical response of both detectors to fibronectin concentration is linear in the range 5-100 ng/mL. The changes in mass on the surface of a quartz crystal covered with a layer of gold during biosensor formation were investigated with a QCM. The equilibrium constant was determined to be K_C = 1.22∙10⁸ dm³/mol and the dissociation constant to be K_D= 8.20 ∙ 10^-9 mol/dm³.

Keywords: Fibronectin; Kinetics of interactions; QCM; SPRi.

MeSH terms

Biosensing Techniques* / methods
Fibronectins
Gold / chemistry
Quartz Crystal Microbalance Techniques / methods
Surface Plasmon Resonance*

Substances

Fibronectins
Gold